Simultaneous determination of six constituents in the fruit of Acanthopanax sessiliflorus (Rupr. et Maxim.) Seem. by HPLC-UV.

A simple and accurate liquid chromatographic method was developed for the simultaneous determination of six constituents in the fruit of Acanthopanax sessiliflorus. The conditions of sample extraction were optimised by using orthogonal design. The method provided good accuracy with recoveries in the range of 95.6-101.6% and good precision with RSD values less than 3.0%, which has been successfully applied to the quantitative determination of the six compounds in the fruit of A. sessiliflorus from two maturation periods.