Di Liu1, Pishan Yang2, Deyu Hu3, Fuxiang Liu4. 1. Dept. of Prosthodontics, Stomatological Hospital of Shandong University, Jinan 250012, China. 2. Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China. 3. State Key Laboratory of Oral Diseases, Dept. of Preventive Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. 4. State Key Laboratory of Oral Diseases, Dept. of Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Abstract
OBJECTIVE: To evaluate the therapeutic effects of 2% minocycline hydrochloride liposome controlled-release gel on the periodontitis in an established rat periodontitis model. METHODS: Biocompatibility was tested by oral perfusion sample solution for long-term observation. Minocycline hydrochloride liposome controlled-release gel was utilized to treat the established rat periodontitis model. The rats were selected randomly and divided into three groups: group A (PERIO-treated group), group B (minocycline hydrochloride liposome controlled-release gel treated group), and group C (negative control group). The gingival index (GI) and probing depth (PD) were detected, and the number of mononuclear and broken bone cells were examined after 7, 14, 28, and 56 d. RESULTS: The minocycline hydrochloride liposome controlled-release gel exhibited excellent biocompatibility based on weight measure and tissue section evaluation. The rats with periodontitis demonstrated that GI, PD, and the number of mononuclear and broken bone cells of group B decreased in 14, 28, and 56 d. Pathological observation showed that new bones and fibers were formed in group B. CONCLUSION: Minocycline hydrochloride liposome controlled-release gel improves rat periodontitis, thereby providing valuable evidence for clinical application.
OBJECTIVE: To evaluate the therapeutic effects of 2% minocycline hydrochloride liposome controlled-release gel on the periodontitis in an established ratperiodontitis model. METHODS: Biocompatibility was tested by oral perfusion sample solution for long-term observation. Minocycline hydrochloride liposome controlled-release gel was utilized to treat the established ratperiodontitis model. The rats were selected randomly and divided into three groups: group A (PERIO-treated group), group B (minocycline hydrochloride liposome controlled-release gel treated group), and group C (negative control group). The gingival index (GI) and probing depth (PD) were detected, and the number of mononuclear and broken bone cells were examined after 7, 14, 28, and 56 d. RESULTS: The minocycline hydrochloride liposome controlled-release gel exhibited excellent biocompatibility based on weight measure and tissue section evaluation. The rats with periodontitis demonstrated that GI, PD, and the number of mononuclear and broken bone cells of group B decreased in 14, 28, and 56 d. Pathological observation showed that new bones and fibers were formed in group B. CONCLUSION:Minocycline hydrochloride liposome controlled-release gel improves ratperiodontitis, thereby providing valuable evidence for clinical application.
Authors: Afroz S Mohammad; Jessica I Griffith; Chris E Adkins; Neal Shah; Emily Sechrest; Emma L Dolan; Tori B Terrell-Hall; Bart S Hendriks; Helen Lee; Paul R Lockman Journal: Pharm Res Date: 2018-01-09 Impact factor: 4.200