Cold-induced sweetening of tissue cultures of Solanum tuberosum L.

Callus cultures derived from tubers of Solanum tuberosum L. cv. "Record" were transferred from a complete medium to a maintenance medium that lacked sugar and were then incubated at 2° or 25°. Incubation at 2° for 5 days led to an accumulation of sucrose. This accumulation did not occur at the expense of stored reducing sugars and was not found at 25°. Such cultures could be used for the study of cold-induced sweetening in potatoes.