Modification of oxygen evolving center by Tris-washing.

Tris-washing inhibits the O2-evolving center of chloroplasts and their particles specifically and reversibly, and it was applied to many investigations on O2-evolving center and PS II reaction center. In this review are introduced the various photosynthetic investigations in which Tris-washing was applied and are also discussed briefly on the site and the mechanism of Tris-inactivation, properties of P680 and Z, characteristic change in fluorescence and delayed light emission, and reactivation of O2-evolving center by DCPIP.H2-treatment and photo-reactivation of Tris-washed chloroplasts and their particles.