Archana Kumari1, Abha Singh1, Ritu Singh2. 1. Department of Obstetrics and Gynaecology, Lady Hardinge Medical College and Associated Hospitals, New Delhi, India. 2. Department of Biochemistry, Lady Hardinge Medical College and Associated Hospitals, New Delhi, India.
Abstract
BACKGROUND: 24-h urine protein is traditionally used as a gold standard method for protein estimation. Because of the operational difficulty, there is the necessity to use rapid, convenient, and reliable method of proteinuria estimation. AIM: We carried out this study to compare the two rapid methods of protein estimation: dipstick method and spot urine protein creatinine ratio (UPCR) with that of 24-h urine protein in patients of preeclampsia with advanced gestational period. METHODOLOGY: The values of proteinuria estimated by dipstick method and spot UPCR were compared with that of 24-h urine protein. The strength of correlation was measured by Pearson's correlation coefficient (r). A p value of <0.05 is considered to be statistically significant. The most discriminant spot UPCR value for detecting significant proteinuria (≥300 mg/day) was determined by plotting receiver-operator curve (ROC). RESULT: The value of spot UPCR strongly correlated with 24-h urine protein (r = 0.88 with p value <0.001). The most discriminant spot UPCR value for detecting significant proteinuria (≥300 mg/day) was 0.3. The estimation of proteinuria by dipstick method was poorly correlated with 24-h urine protein with r = -0.09. CONCLUSION: Spot UPCR can be used as a rapid and reliable alternative method in preference to 24-h proteinuria in patients of preeclampsia of advanced gestational age.
BACKGROUND: 24-h urine protein is traditionally used as a gold standard method for protein estimation. Because of the operational difficulty, there is the necessity to use rapid, convenient, and reliable method of proteinuria estimation. AIM: We carried out this study to compare the two rapid methods of protein estimation: dipstick method and spot urine protein creatinine ratio (UPCR) with that of 24-h urine protein in patients of preeclampsia with advanced gestational period. METHODOLOGY: The values of proteinuria estimated by dipstick method and spot UPCR were compared with that of 24-h urine protein. The strength of correlation was measured by Pearson's correlation coefficient (r). A p value of <0.05 is considered to be statistically significant. The most discriminant spot UPCR value for detecting significant proteinuria (≥300 mg/day) was determined by plotting receiver-operator curve (ROC). RESULT: The value of spot UPCR strongly correlated with 24-h urine protein (r = 0.88 with p value <0.001). The most discriminant spot UPCR value for detecting significant proteinuria (≥300 mg/day) was 0.3. The estimation of proteinuria by dipstick method was poorly correlated with 24-h urine protein with r = -0.09. CONCLUSION: Spot UPCR can be used as a rapid and reliable alternative method in preference to 24-h proteinuria in patients of preeclampsia of advanced gestational age.
Entities:
Keywords:
24 h urine protein; Dipstick method; Preeclampsia; Spot UPCR
Authors: Anne-Marie Côté; Tabassum Firoz; André Mattman; Elaine M Lam; Peter von Dadelszen; Laura A Magee Journal: Am J Obstet Gynecol Date: 2008-08-21 Impact factor: 8.661