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Literature DB >> 24431550

Multiplex PCR assay using SCAR primers to detect Eimeria spp. in chicken.

P Venkateswara Rao¹, M Raman², G Dhinakarraj³, S Abdul Basith², S Gomathinayagam².

Abstract

About 11 faecal samples from various regions of Tamil Nadu and Andhra Pradesh containing mixed spectrum of Eimeria species detected by morphometry viz. 15.4 × 11.2, 28.5 × 20.3, 31.1 × 18.5, 13.2 × 12.4, 20.8 × 17.5 and 22 × 18 μm for E. acervulina, E. brunetti, E. maxima, E. mitis, E. necatrix, E. praecox and E. tenella respectively were taken for the study. The oocysts were concentrated and purified using faecal harvest protocol. The genomic DNA is extracted as per kit protocol. The amplicons of sizes 539 bp (E. tenella) and 460 bp (E. mitis) obtained could be visualised in a single lane for the multiplex PCR assay using sequence characterized amplified region primers.

Entities: Species

Keywords: Eimeria; Faecal harvest; Mixed spectrum; Morphometry; Multiplex PCR; SCAR

Year: 2012 PMID： 24431550 PMCID： PMC3590386 DOI： 10.1007/s12639-012-0142-z

Source DB: PubMed Journal: J Parasit Dis ISSN： 0971-7196

17 in total

1. Single-strand restriction fragment length polymorphism analysis of the second internal transcribed spacer (ribosomal DNA) for six species of Eimeria from chickens in Australia.

Authors: W G Woods; K G Whithear; D G Richards; G R Anderson; W K Jorgensen; R B Gasser
Journal: Int J Parasitol Date: 2000-08 Impact factor: 3.981

10. Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic .Eimeria species of the chicken.

Authors: B E Schnitzler; P L Thebo; J G Mattsson; F M Tomley; M W Shirley
Journal: Avian Pathol Date: 1998 Impact factor: 3.378

1 in total

1. Sporulation dynamics of poultry Eimeria oocysts in Chennai.

Authors: P Venkateswara Rao; M Raman; S Gomathinayagam
Journal: J Parasit Dis Date: 2013-11-26

1 in total

Multiplex PCR assay using SCAR primers to detect Eimeria spp. in chicken.

1. Single-strand restriction fragment length polymorphism analysis of the second internal transcribed spacer (ribosomal DNA) for six species of Eimeria from chickens in Australia.

2. Automated, fluorescence-based approach for the specific diagnosis of chicken coccidiosis.

3. A novel procedure for total nucleic acid extraction from small numbers of Eimeria species oocysts.

4. Eimeria praecox: selection and characteristics of precocious lines.

5. The specific characters of the Eimeria, with special reference to the coccidia of the fowl.

6. Isoenzymes of Eimeria from the domestic fowl: electrophoretic variants among species, strains and clones.

7. PCR identification of chicken Eimeria: a simplified read-out.

Review 8. Problems in the identification of species of Eimeria.

9. Eimeria tenella: characterization of a 5S ribosomal RNA repeat unit and its use as a species-specific probe.

10. Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic .Eimeria species of the chicken.

1. Sporulation dynamics of poultry Eimeria oocysts in Chennai.