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Literature DB >> 24431494

In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.).

Abstract

A system for in vitro selection of drought tolerant callus lines in sugarcane was developed. High molecular weight PEG was used as selective agent. Selected callus line grew better than non-selected callus when grown on different concentrations of PEG. The activity of antioxidant enzymes like CAT, POX, APX and SOD were high in selected callus than in non-selected callus. Osmolytes like proline and ascorbic acid were at higher levels in selected callus than in non-selected callus, however at higher concentrations (20-30 %) of PEG, levels of proline and ascorbic acid decreased. The frequency of organogenesis and number of plantlets decreased in selected callus than in non-selected callus. The results can be used for in vitro screening and manipulations of sugarcane for improvement of drought tolerance.

Entities: Chemical Species

Keywords: Antioxidant enzymes; Callus; PEG; Proline; Selection; Sugarcane

Year: 2013 PMID： 24431494 PMCID： PMC3656189 DOI： 10.1007/s12298-013-0162-x

Source DB: PubMed Journal: Physiol Mol Biol Plants ISSN： 0974-0430

8 in total

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

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1. The effects of osmotic stress on the cell wall-plasma membrane domains of the unicellular streptophyte, Penium margaritaceum.

Authors: David S Domozych; Li Kozel; Kattia Palacio-Lopez
Journal: Protoplasma Date: 2021-04-30 Impact factor: 3.356

1 in total

In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.).

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

2. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions.

3. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation.

4. Proline accumulation and the adaptation of cultured plant cells to water stress.

5. Protein damage and degradation by oxygen radicals. I. general aspects.

6. Analysis of variants affecting the catalase developmental program in maize scutellum.

Review 7. Biological effects of the superoxide radical.

8. Physiological changes in cultured sorghum cells in response to induced water stress : I. Free proline.

1. The effects of osmotic stress on the cell wall-plasma membrane domains of the unicellular streptophyte, Penium margaritaceum.