| Literature DB >> 24430451 |
Yu-Tzu Chang1, Hsuan-Chen Wang, Ming-Cheng Wang, An-Bang Wu, Junne-Ming Sung, H Sunny Sun, Ih-Jen Su, Wei-Chih Kan, Chih-Chiang Chien, Jyh-Chang Hwang, Hsien-Yi Wang, Chin-Chung Tseng, Chi-Jung Wu.
Abstract
PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.Entities:
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Year: 2014 PMID: 24430451 PMCID: PMC3993497 DOI: 10.1128/JCM.03106-13
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948