[The influence of actinomycin D, puromycin, cycloheximide, chloramphenicol, and rifampicin on gamete formation in Acetabularia mediterranea].

Cysts of Acetabularia mediterranea were induced to germinate in the presence of various inhibitors of protein synthesis. Actinomycin D (50 μg/ml), puromycin (10 μg/ml) and cycloheximide (0.1 μg/ml) were found to be strong inhibitors of gamete formation, while chloramphenicol (up to 100 μg/ml) and rifampicin (10 μg/ml) did not show any effect. Malformations were found after treatment with puromycin and very high (200 μg/ml) concentrations of chloramphenicol.