Glycoproteins of GpIbα and GpIIbIIIa on the Synthetic or Naturally Occurred Platelet-Derived Microparticles.

Infusible platelet membranes (IPM) prepared from new or outdated human platelets have been developed as an alternative to standard platelet concentrates (PCs), with the additional advantage of long shelf life and increased viral safety. Lack of gpIIb/IIIa has been reported as one of the properties of the IPM microparticles. In re-examining this issue, we studied the molecules of gpIIb/IIIa and gpIbα on the surface of IPM microparticles. These molecules could better evaluate the functional efficacy of these microparticles. In comparison, we also surveyed the expression of these molecules on the surface of the naturally occurred platelet-derived microparticles during 7 days storage of PC. Unlike the previous reports, the results of this study illustrated that the produced IPM retained gpIIb/IIIa molecules. Besides, gpIbα and gpIIb/IIIa were also present on the nPMPs and their levels were significantly decreased during 7 days storage of PCs (P value = 0.001).