Literature DB >> 24425390

Production of pectolytic enzymes fromErwinia grown on different carbon sources.

V E Shevchik1, A N Evtushenkov, H V Babitskaya, Y K Fomichev.   

Abstract

A quantitative analysis of pectolytic enzymes (polygalacturonase (PG), pectin methyl esterase (PME) and six isoenzymes of pectate lyase (PL)) produced byErwinia bacteria in the presence of diverse carbon sources was made by preparative electrophoresis. Synthesis of each of these enzymes was regulated independently; different induction and repression ratios (about 10- to 1000-fold) were observed for diverse PL isoenzymes, PG and PME. The possibility of using specially constructed media for the production of pectinase complexes with a specific spectra of pectolytic enzymes has been demonstrated.

Entities:  

Year:  1992        PMID: 24425390     DOI: 10.1007/BF01195828

Source DB:  PubMed          Journal:  World J Microbiol Biotechnol        ISSN: 0959-3993            Impact factor:   3.312


  11 in total

1.  Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

Authors:  R A REISFELD; U J LEWIS; D E WILLIAMS
Journal:  Nature       Date:  1962-07-21       Impact factor: 49.962

2.  [Cloning of pectate-lyase genes of Erwinia chrysanthemi in Escherichia coli cells].

Authors:  A N Evtushenkov; V E Shevchik; Iu K Fomichev
Journal:  Mol Gen Mikrobiol Virusol       Date:  1986-04

3.  Synthesis and excretion of polygalacturonic acid trans-eliminase in Erwinia, Yersinia, and Klebsiella species.

Authors:  A K Chatterjee; G E Buchanan; M K Behrens; M P Starr
Journal:  Can J Microbiol       Date:  1979-01       Impact factor: 2.419

4.  Extracellular and intracellular polygllacturonic acid trans-eliminases of Erwinia carotovora.

Authors:  F Moran; S Nasuno; M P Starr
Journal:  Arch Biochem Biophys       Date:  1968-02       Impact factor: 4.013

5.  Impaired induction and self-catabolite repression of extracellular pectate lyase in Erwinia chrysanthemi mutants deficient in oligogalacturonide lyase.

Authors:  A Collmer; D F Bateman
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205

6.  Regulation of expression of pectate lyase genes pelA, pelD, and pelE in Erwinia chrysanthemi.

Authors:  S Reverchon; J Robert-Baudouy
Journal:  J Bacteriol       Date:  1987-06       Impact factor: 3.490

7.  Cloning of genes encoding pectolytic enzymes from a genomic library of the phytopathogenic bacterium, Erwinia chrysanthemi.

Authors:  S Reverchon; N Hugouvieux-Cotte-Pattat; J Robert-Baudouy
Journal:  Gene       Date:  1985       Impact factor: 3.688

8.  Effect of pectinase on rumen fermentation in sheep and lambs.

Authors:  M Baran; V Kmet
Journal:  Arch Tierernahr       Date:  1987 Jul-Aug

9.  Molecular cloning of pectate lyase genes from Erwinia chrysanthemi and their expression in Escherichia coli.

Authors:  N T Keen; D Dahlbeck; B Staskawicz; W Belser
Journal:  J Bacteriol       Date:  1984-09       Impact factor: 3.490

10.  Molecular cloning of Erwinia chrysanthemi pectinase and cellulase structural genes.

Authors:  A Kotoujansky; A Diolez; M Boccara; Y Bertheau; T Andro; A Coleno
Journal:  EMBO J       Date:  1985-03       Impact factor: 11.598

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