| Literature DB >> 24423269 |
Jagpreet S Nanda1, Jon R Lorsch2.
Abstract
N-hydroxysuccinimde (NHS) ester-mediated derivitization involves the reaction of this amine-reactive group with the primary amines of a protein or a biomolecule. Using NHS chemistry allows one to conjugate various fluorescent probes, biotin, and cross-linkers to primary amines. For example, we use NHS ester chemistry to fluorescently label the amino terminus of a protein with the dye, 5-(and-6)-carboxyfluorescein, succinimidyl ester (5(6)-FAM, SE).Entities:
Keywords: Efficiency of labeling calculation; Fluorescent labeling of protein; Fluorophores; Lyophilized protein; NHS ester derivitization; UV spectrophotometer
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Year: 2014 PMID： 24423269 DOI： 10.1016/B978-0-12-420070-8.00008-8
Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600