Literature DB >> 24421132

Purification and properties of mannanase from Bacillus subtilis.

N S Mendoza1, M Arai, T Kawaguchi, T Yoshida, L M Joson.   

Abstract

Extracellular mannanase from Bacillus subtilis NM-39, an isolate from Philippine soil, was purified about 240-fold with a yield of 7.3% by ammonium sulphate fractionation, DEAE-Toyopearl chromatography and Sephacryl S-200 gel filtration. Its M r was 38 kDa and it had a pI of 4.8 and optimum activity at pH 5.0 and 55°C. It was stable at pH 4 to 9 and below 55°C. The amino acid composition of the enzyme was in the order Gly>Glx>Ser and Asx>Ala.

Entities:  

Year:  1994        PMID： 24421132     DOI： 10.1007/BF00367665

Source DB:  PubMed          Journal:  World J Microbiol Biotechnol        ISSN： 0959-3993            Impact factor:   3.312


  8 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  The beta-mannanase from "Caldocellum saccharolyticum" is part of a multidomain enzyme.

Authors:  M D Gibbs; D J Saul; E Lüthi; P L Bergquist
Journal:  Appl Environ Microbiol       Date:  1992-12       Impact factor: 4.792

3.  Notes on sugar determination.

Authors:  M SMOGYI
Journal:  J Biol Chem       Date:  1952-03       Impact factor: 5.157

4.  Studies on the structure of yeast mannan. I. Purification and some properties of an alpha-mannosidase from an arthrobacter species.

Authors:  G H Jones; C E Ballou
Journal:  J Biol Chem       Date:  1969-02-10       Impact factor: 5.157

5.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

Review 6.  Hemicellulases: their occurrence, purification, properties, and mode of action.

Authors:  R F Dekker; G N Richards
Journal:  Adv Carbohydr Chem Biochem       Date:  1976       Impact factor: 12.200

7.  Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins.

Authors:  O Vesterberg; H Svensson
Journal:  Acta Chem Scand       Date:  1966

8.  Isolation of mannan-utilizing bacteria and the culture conditions for mannanase production.

Authors:  N S Mendoza; M Arai; T Kawaguchi; F S Cubol; E G Panerio; T Yoshida; L M Joson
Journal:  World J Microbiol Biotechnol       Date:  1994-01       Impact factor: 3.312
  8 in total
  6 in total

1.  Characterization, gene cloning, and heterologous expression of β-mannanase from a thermophilic Bacillus subtilis.

Authors:  Pijug Summpunn; Suttidarak Chaijan; Duangnate Isarangkul; Suthep Wiyakrutta; Vithaya Meevootisom
Journal:  J Microbiol       Date:  2011-03-03       Impact factor: 3.422

2.  Biochemical characterization of a thermostable endomannanase/endoglucanase from Dictyoglomus turgidum.

Authors:  Francesca Anna Fusco; Raffaele Ronca; Gabriella Fiorentino; Emilia Pedone; Patrizia Contursi; Simonetta Bartolucci; Danila Limauro
Journal:  Extremophiles       Date:  2017-11-25       Impact factor: 2.395

3.  Purification, characterization, and overexpression of an endo-1,4-β-mannanase from thermotolerant Bacillus sp. SWU60.

Authors:  Weeranuch Seesom; Polphet Thongket; Tomohiro Yamamoto; Shigeo Takenaka; Tatsuji Sakamoto; Wasana Sukhumsirichart
Journal:  World J Microbiol Biotechnol       Date:  2017-02-20       Impact factor: 3.312

4.  Characterization of the Bacillus subtilis WL-3 mannanase from a recombinant Escherichia coli.

Authors:  Ki-Hong Yoon; Seesub Chung; Byung-Lak Lim
Journal:  J Microbiol       Date:  2008-07-05       Impact factor: 3.422

5.  Expression, homology modeling and enzymatic characterization of a new β-mannanase belonging to glycoside hydrolase family 1 from Enterobacter aerogenes B19.

Authors:  Siyu Liu; Tangbing Cui; Yan Song
Journal:  Microb Cell Fact       Date:  2020-07-14       Impact factor: 5.328

6.  Characterization and high-efficiency secreted expression in Bacillus subtilis of a thermo-alkaline β-mannanase from an alkaliphilic Bacillus clausii strain S10.

Authors:  Cheng Zhou; Yanfen Xue; Yanhe Ma
Journal:  Microb Cell Fact       Date:  2018-08-11       Impact factor: 5.328
  6 in total

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