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Literature DB >> 24421082

Intracellular localization of glutamine synthetase in Chlorogloeopsis fritschii.

Abstract

After differential centrifugation of cell-free extracts of Chlorogloeopsis fritschii, 71% of the original glutamine synthetase (GS) activity was associated with the thylakoids, while little activity was detected in the cytoplasmic membranes. Monospecific antiserum to a purified GS inhibited 88% of the enzyme activity in solubilized thylakoid membranes. An antiserum raised against thylakoids gave 81% inhibition. However, using intact thylakoid membranes, only 7% inhibition was obtained with the GS antiserum, indicating that GS is located inside the thylakoid membranes.

Entities: Disease Species

Year: 1994 PMID： 24421082 DOI： 10.1007/BF00144457

Source DB: PubMed Journal: World J Microbiol Biotechnol ISSN： 0959-3993 Impact factor: 3.312

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References

11 in total

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Authors: D C Yoch; M Cantu; Z M Zhang
Journal: J Bacteriol Date: 1983-05 Impact factor: 3.490

Intracellular localization of glutamine synthetase in Chlorogloeopsis fritschii.

1. Immuno-gold localization of glutamine synthetase in a nitrogen-fixing cyanobacterium (Anabaena cylindrica).

2. Purification and Biochemical Analysis of the Cytoplasmic Membrane from the Desiccation-Tolerant Cyanobacterium Nostoc commune UTEX 584.

3. Glycollate oxidation by thylakoids of the cyanobacteria Anabaena cylindrica, Nostoc muscorum and Chlorogloea fritschii.

Review 4. Some aspects of structure and function in N2-fixing cyanobacteria.

5. The intracellular localization of the glycollate-oxidising enzyme of Anabaena cylindrica.

Review 6. Respiration and photosynthesis in energy-transducing membranes of cyanobacteria.

7. Purification and properties of unicellular blue-green algae (order Chroococcales).

8. Intracellular localization of glycolate dehydrogenase in a blue-green alga.

9. Studies on the dependence of chlorophyll synthesis on protein synthesis in Euglena gracilis, together with a nomogram for determination of chlorophyll concentration.

10. Evidence for a glutamine synthetase-chromatophore association in the phototroph Rhodospirillum rubrum: purification, properties, and regulation of the enzyme.