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Literature DB >> 24420881

Isolation and characterization of restriction endonuclease EclHKI from an Enterobacter cloacae strain.

H Y Chan¹, Y C Chan, K M Kam, P C Shaw.
1. , .

Abstract

An Enterobacter cloacae strain, producing restriction enzyme EclHKI, was isolated from a decaying potato. The enzyme is an isoschizomer of Eam1105I, which recognizes and cleaves GACNNN!NNGTC. EclHKI was produced at high activity (4×10(4) U/g wet cells) and was purified from contaminants which interfere with restriction digestion by passing the cell lysate through DEAE-Sephacel and heparin columns. Activity was optimal at 37°C in a medium salt buffer.

Entities: Chemical Species

Year: 1994 PMID： 24420881 DOI： 10.1007/BF00357558

Source DB: PubMed Journal: World J Microbiol Biotechnol ISSN： 0959-3993 Impact factor: 3.312

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References

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9 in total

Isolation and characterization of restriction endonuclease EclHKI from an Enterobacter cloacae strain.

1. Restriction endonuclease from thermophilic bacterial species III. Isolation and characterization of BsiHKA I.

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