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Literature DB >> 24420012

Chlorophyll interference with phytochrome measurement.

Abstract

Measurements of phytochrome by Δ (ΔA725-815 nm) were completely suppressed at chlorophyll concentrations of the order of 20-40 μg g(-1) f.wt. in vivo and 37 μg cm(-3) in vitro, and the readings were reduced by 50% at only 12 μg cm(-3) in vitro. At these concentrations of chlorophyll in aqueous methanol, the loss of phytochrome signal in vitro appeared to be due to failure of phytochrome photoconversion rather than to interference with ΔA measuremebt by chlorophyll fluorescence in the 125/815 nm measuring beam.

Entities: Chemical Mutation

Year: 1977 PMID： 24420012 DOI： 10.1007/BF00387159

Source DB: PubMed Journal: Planta ISSN： 0032-0935 Impact factor: 4.116

4 in total

7 in total

1. The influence of light quality on the phytochrome content of light grown Sinapis alba L. and Phaseolus aureus Roxb.

Authors: C A Kilsby; C B Johnson
Journal: Planta Date: 1981-10 Impact factor: 4.116

2. Phytochrome in green tissue: Spectral and immunochemical evidence for two distinct molecular species of phytochrome in light-grown Avena sativa L.

Authors: J G Tokuhisa; S M Daniels; P H Quail
Journal: Planta Date: 1985-06 Impact factor: 4.116

3. Partial purification and initial characterization of phytochrome from the mossAtrichum undulatum P. Beauv. grown in the light.

Authors: P Lindemann; S E Braslavsky; E Hartmann; K Schaffner
Journal: Planta Date: 1989-12 Impact factor: 4.116

7. Light requirement, phytochrome and photoperiodic induction of flowering of Pharbits nil Chois : II. A critical examination of spectrophotometric assays of phytochrome transformations.

Authors: D Vince-Prue; R W King; P H Quail
Journal: Planta Date: 1978-01 Impact factor: 4.116

7 in total

Chlorophyll interference with phytochrome measurement.

1. The influence of chlorophyll on in-vivo difference spectra of phytochrome.

2. Influence of chlorophyll content on phytochrome measurements in turnip cotyledons.

3. Reversible redistribution of phytochrome within the cell upon conversion to its physiologically active form.

4. An immunochemical characterization of the phytochrome destruction reaction.

1. The influence of light quality on the phytochrome content of light grown Sinapis alba L. and Phaseolus aureus Roxb.

2. Phytochrome in green tissue: Spectral and immunochemical evidence for two distinct molecular species of phytochrome in light-grown Avena sativa L.

3. Partial purification and initial characterization of phytochrome from the mossAtrichum undulatum P. Beauv. grown in the light.

4. Light-induced changes in the photoresponses of plant stems the loss of a high irradiance response to far-red light.

5. Spectrophotometric phytochrome measurements in light-grown Avena sativa L.

6. Distorted phytochrome action spectra in green plants.

7. Light requirement, phytochrome and photoperiodic induction of flowering of Pharbits nil Chois : II. A critical examination of spectrophotometric assays of phytochrome transformations.