Literature DB >> 24419911

Assaying for pyruvate, orthophosphate dikinase activity: Necessary precautions with phosphoenolpyruvate carboxylase as coupling enzyme.

G Salahas1, Y Manetas, N A Gavalas.   

Abstract

Phosphoenolpyruvate carboxylase (EC 4.1.1.31), used as a coupling enzyme in the assay of the pyruvate, orthophosphate dikinase (EC 2.7.9.1) forward reaction, is a serious limiting factor for the overall rate when added at a level of 0.2-0.3 unit/ml of assay medium. Nonlimiting assay conditions are obtained by either increasing the level of the coupling enzyme to 3 units/ml or adding 6mM glucose-6-phosphate as an activator/stabilizer of phosphoenolpyruvate carboxylase.

Entities:  

Year:  1990        PMID： 24419911     DOI： 10.1007/BF00032598

Source DB:  PubMed          Journal:  Photosynth Res        ISSN： 0166-8595            Impact factor:   3.573


  13 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Artifacts in the assay of maize leaf phosphoenolpyruvate carboxylase activity due to its instability.

Authors:  K Angelopoulos; K Stamatakis; Y Manetas; N A Gavalas
Journal:  Photosynth Res       Date:  1988-11       Impact factor: 3.573

3.  Light and temperature dependence of the rate and degree of activation of pyruvate, Pi dikinase in vivo in maize.

Authors:  G E Edwards; M Ujihira; T Sugiyama
Journal:  Photosynth Res       Date:  1980-09       Impact factor: 3.573

4.  Activation of NADP-Malate Dehydrogenase, Pyruvate,Pi Dikinase, and Fructose 1,6-Bisphosphatase in Relation to Photosynthetic Rate in Maize.

Authors:  H Usuda; M S Ku; G E Edwards
Journal:  Plant Physiol       Date:  1984-09       Impact factor: 8.340

5.  Pyruvate orthophosphate dikinase of c(3) seeds and leaves as compared to the enzyme from maize.

Authors:  K Aoyagi; J A Bassham
Journal:  Plant Physiol       Date:  1984-06       Impact factor: 8.340

6.  Regulation of C4 photosynthesis: mechanism of activation and inactivation of extracted pyruvate, inorganic phosphate dikinase in relation to dark/light regulation.

Authors:  K S Chapman; M D Hatch
Journal:  Arch Biochem Biophys       Date:  1981-08       Impact factor: 4.013

7.  Properties and reaction mechanism of C4 leaf pyruvate,Pi dikinase.

Authors:  C L Jenkins; M D Hatch
Journal:  Arch Biochem Biophys       Date:  1985-05-15       Impact factor: 4.013

8.  Role of Metabolites in the Reversible Light Activation of Pyruvate, Orthophosphate Dikinase in Zea mays Mesophyll Cells in Vivo.

Authors:  C A Roeske; R Chollet
Journal:  Plant Physiol       Date:  1989-05       Impact factor: 8.340

9.  Regulation of C4 photosynthesis: inactivation of pyruvate, Pi dikinase by ADP-dependent phosphorylation and activation by phosphorolysis.

Authors:  A R Ashton; J N Burnell; M D Hatch
Journal:  Arch Biochem Biophys       Date:  1984-05-01       Impact factor: 4.013

10.  A new enzyme for the interconversion of pyruvate and phosphopyruvate and its role in the C4 dicarboxylic acid pathway of photosynthesis.

Authors:  M D Hatch; C R Slack
Journal:  Biochem J       Date:  1968-01       Impact factor: 3.857
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  6 in total

1.  Comparative transcriptional profiling and preliminary study on heterosis mechanism of super-hybrid rice.

Authors:  Gui-Sheng Song; Hong-Li Zhai; Yong-Gang Peng; Lei Zhang; Gang Wei; Xiao-Ying Chen; Yu-Guo Xiao; Lili Wang; Yue-Jun Chen; Bin Wu; Bin Chen; Yu Zhang; Hua Chen; Xiu-Jing Feng; Wan-Kui Gong; Yao Liu; Zhi-Jie Yin; Feng Wang; Guo-Zhen Liu; Hong-Lin Xu; Xiao-Li Wei; Xiao-Ling Zhao; Pieter B F Ouwerkerk; Thomas Hankemeier; Theo Reijmers; Rob van der Heijden; Cong-Ming Lu; Mei Wang; Jan van der Greef; Zhen Zhu
Journal:  Mol Plant       Date:  2010-08-20       Impact factor: 13.164

2.  Effects of glycerol on the in vitro stability and regulatory activation/inactivation of pyruvate, orthophosphate dikinase of Zea mays L.

Authors:  G Salahas; Y Manetas; N A Gavalas
Journal:  Photosynth Res       Date:  1990-10       Impact factor: 3.573

3.  Kalanchoë PPC1 Is Essential for Crassulacean Acid Metabolism and the Regulation of Core Circadian Clock and Guard Cell Signaling Genes.

Authors:  Susanna F Boxall; Nirja Kadu; Louisa V Dever; Jana Kneřová; Jade L Waller; Peter J D Gould; James Hartwell
Journal:  Plant Cell       Date:  2020-02-12       Impact factor: 11.277

4.  Small-molecule inhibition of pyruvate phosphate dikinase targeting the nucleotide binding site.

Authors:  Alexander Minges; Georg Groth
Journal:  PLoS One       Date:  2017-07-10       Impact factor: 3.240

5.  Structural intermediates and directionality of the swiveling motion of Pyruvate Phosphate Dikinase.

Authors:  Alexander Minges; Daniel Ciupka; Christian Winkler; Astrid Höppner; Holger Gohlke; Georg Groth
Journal:  Sci Rep       Date:  2017-03-30       Impact factor: 4.379

6.  Establishing Trypanosoma cruzi farnesyl pyrophosphate synthase as a viable target for biosensor driven fragment-based lead discovery.

Authors:  Giulia Opassi; Helena Nordström; Arne Lundin; Valeria Napolitano; Francesca Magari; Tom Dzus; Gerhard Klebe; U Helena Danielson
Journal:  Protein Sci       Date:  2020-02-07       Impact factor: 6.725
  6 in total

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