The polyadenylic sequences in the ribonucleic acid of the fern Anemia phyllitidis.

The vegetative prothalli (1-3 weeks old) of Anemia were incubated for 24 h in [(14)C]adenine. The RNA was phenol extracted from whole cells and the poly (A) sequences were isolated by nuclease digestion followed by poly (U)-sepharose chromatography. About 2-3% of the total radioactivity was retained on the column. The base composition of the nuclease resistant RNA was: C, 1.4; G, 3.6; A, 93.3; and U, 1.7. It is concluded that Anemia RNA contains poly adenylate sequences.