| Literature DB >> 24418552 |
Evandra Strazza Rodrigues1, Mayra Dorigan de Macedo1, Mariana Tomazini Pinto1, Maristela Delgado Orellana2, Maurício Cristiano Rocha Junior1, Danielle Aparecida Rosa de Magalhães3, Yuetsu Tanaka4, Osvaldo Massaiti Takayanagui5, Dimas Tadeu Covas2, Simone Kashima6.
Abstract
The typical characteristics of mesenchymal stem cells (MSCs) can be affected by inflammatory microenvironment; however, the exact contribution of HTLV-1 to MSC dysfunction remains to be elucidated. In this study, we demonstrated that MSC cell surface molecules VCAM-1 and ICAM-1 are upregulated by contact with HTLV-1, and HLA-DR was most highly expressed in MSCs co-cultured with MT2 cells. The expression levels of VCAM-1 and HLA-DR were increased in MSCs cultured in the presence of PBMCs isolated from HTLV-1-infected symptomatic individuals compared with those cultured with cells from asymptomatic infected individuals or healthy subjects. HTLV-1 does not impair the MSC differentiation process into osteocytes and adipocytes. In addition, MSCs were efficiently infected with HTLV-1 in vitro through direct contact with HTLV-1-infected cells; however, cell-free virus particles were not capable of causing infection. In summary, HTLV-1 can alter MSC function, and this mechanism may contribute to the pathogenesis of this viral infection.Entities:
Keywords: ACTB; ATLL; BM; CD105; CD106; CD13; CD271; CD45; CD73; CD90; CMV; ENG, endoglin; GAPDH; GLUT1; HAC; HAM/TSP; HHV; HLA-ABC; HLA-class I; HSPG2; HTLV asymptomatic carriers; HTLV-1; HTLV-associated myelopathy/tropical spastic paraparesis; Human mesenchymal stromal cells; MSC; MSC differentiation; MSC virus infection; NGFR, nerve growth factor receptor; NRP1; NT5E, 5-nucleotidase, ecto; PBMC; PPARγ; RUNX-2; THY1, Thy-1 cell surface antigen; URE; VCAM1, vascular cell adhesion molecule 1; adult T-cell leukemia/lymphoma; aminopeptidase-N; beta-actin; bone marrow; cytomegalovirus; expression relative units; glyceraldehyde-3-phosphate dehydrogenase; heparan sulfate proteoglycan 2; human herpesvirus, HHV8; human major histocompatibility complex (MHC) class I, HLA-A, B, C; human major histocompatibility complex, class I; leukocyte common; mesenchymal stromal cells; neuropilins 1; peripheral blood mononuclear cells; peroxisome proliferator-activated receptors gamma; runt-related transcription factor-2; solute carrier family 2 (facilitated glucose transporter), member 1
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Year: 2013 PMID： 24418552 DOI： 10.1016/j.virol.2013.11.022
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616