Aneesa Ansari1, Md Shahed Zaman Shahriar2, Md Mehedi Hassan3, Shukla Rani Das4, Begum Rokeya5, Md Anwarul Haque2, Md Enamul Haque6, Nirupam Biswas7, Tama Sarkar8. 1. Department of Genetic Engineering and Biotechnology, Jessore Science and Technology University, Jessore, Bangladesh. 2. Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh. 3. Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh; Department of Biomedical Science, Chonbuk National University Medical School, Jeonju 561-712, South Korea. 4. Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science & Technology University, Dinajpur, Bangladesh. 5. Department of Biochemistry and Cell Biology, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM), Dhaka, Bangladesh. 6. Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh. 7. Department of Nutrition and Food Sciences, University of Huddersfield, Huddersfiled, HD1 3DH, United Kingdom. Electronic address: nirupamgene@gmail.com. 8. Department of Applied Nutrition and Food Technology, Islamic University, Kushtia, Bangladesh.
Abstract
OBJECTIVE: To evaluate the antidiabetic and antioxidant potential of Emblica officinalis (E. officinalis) fruit on normal and type 2 diabetic rats. METHODS: Type 2 diabetes was induced into the male Long-Evans rats. The rats were divided into nine groups including control groups receiving water, type 2 diabetic controls, type 2 diabetic rats treated with glibenclamide (T2GT) and type 2 diabetic rats treated with aqueous extract of fruit pulp of E. officinalis. They were fed orally for 8 weeks with a single feeding. Blood was collected by cutting the tail tip on 0 and 28 days and by decapitation on 56 day. Packed red blood cells and serum were used for evaluating different biochemical parameters. RESULTS: Four weeks administration of aqueous extract of E. officinalis improved oral glucose tolerance in type 2 rats and after 8 weeks it caused significant (P<0.007) reduction in fasting serum glucose level compared to 0 day. Triglycerides decreased by 14% but there was no significant change in serum ALT, creatinine, cholesterol and insulin level in any group. Furthermore, reduced erythrocyte malondialdehyde level showed no significant change (P<0.07) but reduced glutathione content was found to be increased significantly (P<0.05). CONCLUSIONS: The aqueous extract of E. officinalis has a promising antidiabetic and antioxidant properties and may be considered for further clinical studies in drug development.
OBJECTIVE: To evaluate the antidiabetic and antioxidant potential of Emblica officinalis (E. officinalis) fruit on normal and type 2 diabeticrats. METHODS: Type 2 diabetes was induced into the male Long-Evans rats. The rats were divided into nine groups including control groups receiving water, type 2 diabetic controls, type 2 diabeticrats treated with glibenclamide (T2GT) and type 2 diabeticrats treated with aqueous extract of fruit pulp of E. officinalis. They were fed orally for 8 weeks with a single feeding. Blood was collected by cutting the tail tip on 0 and 28 days and by decapitation on 56 day. Packed red blood cells and serum were used for evaluating different biochemical parameters. RESULTS: Four weeks administration of aqueous extract of E. officinalis improved oral glucose tolerance in type 2 rats and after 8 weeks it caused significant (P<0.007) reduction in fasting serum glucose level compared to 0 day. Triglycerides decreased by 14% but there was no significant change in serum ALT, creatinine, cholesterol and insulin level in any group. Furthermore, reduced erythrocyte malondialdehyde level showed no significant change (P<0.07) but reduced glutathione content was found to be increased significantly (P<0.05). CONCLUSIONS: The aqueous extract of E. officinalis has a promising antidiabetic and antioxidant properties and may be considered for further clinical studies in drug development.
Authors: Maryam Gul; Zhi-Wei Liu; Roshina Rabail; Fatima Faheem; Noman Walayat; Asad Nawaz; Muhammad Asim Shabbir; Paulo E S Munekata; José M Lorenzo; Rana Muhammad Aadil Journal: Antioxidants (Basel) Date: 2022-04-22