PURPOSE: Malignant mesothelioma is a neoplastic disease linked to asbestos exposure whose diagnosis is limited, so detection methods for an early diagnosis and treatment result essential. Here, we compared proteomic profiles of malignant pleural mesothelioma (MPM) and benign biopsies to search potential biomarkers useful in differential diagnosis. EXPERIMENTAL DESIGN: Tissue biopsies were obtained from 53 patients who were subjected to a diagnostic thoracoscopy. 2DE/MS based approach was used for proteomic analysis and protein validation was carried out by Western blot analysis versus benign and lung carcinoma samples. RESULTS: Among the proteins identified we confirmed known MPM biomarkers such as calretinin and suggested the new ones as prelamin A/C, desmin, vimentin, calretinin, fructose-bisphosphate aldolase A, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, myosin light chain 3 and myosin light chain 6B. Ingenuity software was used to identify the biological processes to which these proteins belong and to construct a potential network. CONCLUSIONS AND CLINICAL RELEVANCE: Overall, our results suggest potential biomarkers that can be useful in occupational medicine for the early identification of the onset of disease in health surveillance of past asbestos-exposed workers, for monitoring the progress of disease and for assessing the response to treatment.
PURPOSE:Malignant mesothelioma is a neoplastic disease linked to asbestos exposure whose diagnosis is limited, so detection methods for an early diagnosis and treatment result essential. Here, we compared proteomic profiles of malignant pleural mesothelioma (MPM) and benign biopsies to search potential biomarkers useful in differential diagnosis. EXPERIMENTAL DESIGN: Tissue biopsies were obtained from 53 patients who were subjected to a diagnostic thoracoscopy. 2DE/MS based approach was used for proteomic analysis and protein validation was carried out by Western blot analysis versus benign and lung carcinoma samples. RESULTS: Among the proteins identified we confirmed known MPM biomarkers such as calretinin and suggested the new ones as prelamin A/C, desmin, vimentin, calretinin, fructose-bisphosphate aldolase A, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, myosin light chain 3 and myosin light chain 6B. Ingenuity software was used to identify the biological processes to which these proteins belong and to construct a potential network. CONCLUSIONS AND CLINICAL RELEVANCE: Overall, our results suggest potential biomarkers that can be useful in occupational medicine for the early identification of the onset of disease in health surveillance of past asbestos-exposed workers, for monitoring the progress of disease and for assessing the response to treatment.
Authors: Veronica Seccia; Elena Navari; Elena Donadio; Claudia Boldrini; Federica Ciregia; Maurizio Ronci; Antonio Aceto; Iacopo Dallan; Antonio Lucacchini; Augusto Pietro Casani; Maria Rosa Mazzoni; Laura Giusti Journal: Head Neck Pathol Date: 2019-05-16
Authors: A Bonotti; R Foddis; S Landi; O Melaiu; C De Santi; L Giusti; E Donadio; F Ciregia; M R Mazzoni; A Lucacchini; M Bovenzi; M Comar; E Pantani; A Pistelli; A Cristaudo Journal: Dis Markers Date: 2017-02-28 Impact factor: 3.434
Authors: Graziano Cugno; José R Parreira; Enea Ferlizza; Lorenzo E Hernández-Castellano; Mariana Carneiro; Jenny Renaut; Noemí Castro; Anastasio Arguello; Juan Capote; Alexandre M O Campos; André M Almeida Journal: PLoS One Date: 2016-03-31 Impact factor: 3.240
Authors: Laura Giusti; Federica Ciregia; Alessandra Bonotti; Ylenia Da Valle; Elena Donadio; Claudia Boldrini; Rudy Foddis; Gino Giannaccini; Maria R Mazzoni; Pier Aldo Canessa; Alfonso Cristaudo; Antonio Lucacchini Journal: EuPA Open Proteom Date: 2016-01-16