| Literature DB >> 24415473 |
Abstract
Despite possible drawbacks (intraspecific polymorphisms and possible fungal contamination), sequencing of the ribosomal RNA gene ITS region remains one of the most popular nuclear sequences used for plant taxonomy and phylogeny. A protocol for PCR amplification and sequencing of this region using universal plant primers is provided.Mesh:
Substances:
Year: 2014 PMID: 24415473 DOI: 10.1007/978-1-62703-767-9_7
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745