Short communication: Production of L-glutamate oxidase by Streptomyces sp. N1 in submerged fermentation.

In submerged fermentation of Streptomyces sp. N1 in a shake flask, glucose (3% w/v) and (NH4)2SO4 (0.6% w/v) were found to be suitable for extracellular L-glutamate oxidase (GluOx) (EC.1.4.3.11) production. GluOx production was higher with the addition of further KCl or MgCl2 to the medium within the range of 0 to 0.12% (w/v). The effect of inoculum type, that is, spore inoculation or mycelium inoculation on GluOx biosynthesis was also investigated, and the maximum GluOx production obtained was 2.7 U/ml after 33h fermentation with mycelium inoculation. The results demonstrated a much higher GluOx production and productivity compared with those reported previously.