Literature DB >> 24415376

Polymerase chain Reaction in molecular biotechnology; appropriate technology for developing countries.

A E Felice1, C Alshinawi.   

Abstract

The product of the Polymerase Chain Reaction (PCR) may be generically suitable for four types of investigations: Discovery PCR, Analytical PCR, Modification by PCR, and Synthetic PCR. Despite the potential problem of contamination with extraneous DNA, PCR is relatively simple and inexpensive, and thereby offers opportunities for laboratories from smaller or developing countries to invest limited resources in competitive global research and development, or in applications of economic and social value in health, agriculture and other sectors.

Year:  1996        PMID: 24415376     DOI: 10.1007/BF00419459

Source DB:  PubMed          Journal:  World J Microbiol Biotechnol        ISSN: 0959-3993            Impact factor:   3.312


  24 in total

Review 1.  Recent advances in the polymerase chain reaction.

Authors:  H A Erlich; D Gelfand; J J Sninsky
Journal:  Science       Date:  1991-06-21       Impact factor: 47.728

Review 2.  A biochemical perspective of the polymerase chain reaction.

Authors:  W Bloch
Journal:  Biochemistry       Date:  1991-03-19       Impact factor: 3.162

3.  Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction.

Authors:  M Orita; Y Suzuki; T Sekiya; K Hayashi
Journal:  Genomics       Date:  1989-11       Impact factor: 5.736

4.  Shedding light on PCR contamination.

Authors:  G Sarkar; S S Sommer
Journal:  Nature       Date:  1990-01-04       Impact factor: 49.962

5.  Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).

Authors:  M Kammann; J Laufs; J Schell; B Gronenborn
Journal:  Nucleic Acids Res       Date:  1989-07-11       Impact factor: 16.971

6.  A common language for physical mapping of the human genome.

Authors:  M Olson; L Hood; C Cantor; D Botstein
Journal:  Science       Date:  1989-09-29       Impact factor: 47.728

7.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

8.  Optimization strategies for the polymerase chain reaction.

Authors:  J F Williams
Journal:  Biotechniques       Date:  1989 Jul-Aug       Impact factor: 1.993

9.  Detection of single base substitutions in total genomic DNA.

Authors:  R M Myers; N Lumelsky; L S Lerman; T Maniatis
Journal:  Nature       Date:  1985 Feb 7-13       Impact factor: 49.962

10.  Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile.

Authors:  T D Brock; H Freeze
Journal:  J Bacteriol       Date:  1969-04       Impact factor: 3.490

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