Literature DB >> 24415230

Rapid and simple assay for feruloyl and p-coumaroyl esterases.

F H O'Neill1, L P Christov, P J Botes, B A Prior.   

Abstract

A rapid, simple and sensitive method for detection of ferulic and p-coumaric acids using HPLC has been developed which can be used to determine the respective phenolic acid esterase activities of microorganisms. Prior concentration, purification or derivatization of the samples are not required. As little as 0.5 mg ferulic or p-coumaric acid/I could be detected and estimated in < 1 h. The method is specific for the two phenolic acids sice no interference by other components was observed.

Entities:  

Year:  1996        PMID： 24415230     DOI： 10.1007/BF00360920

Source DB:  PubMed          Journal:  World J Microbiol Biotechnol        ISSN： 0959-3993            Impact factor:   3.312


  7 in total

1.  Synthesis of methyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside and its use as a substrate to assess feruloyl esterase activity.

Authors:  R D Hatfield; R F Helm; J Ralph
Journal:  Anal Biochem       Date:  1991-04       Impact factor: 3.365

2.  Production by Streptomyces viridosporus T7A of an Enzyme Which Cleaves Aromatic Acids from Lignocellulose.

Authors:  P K Donnelly; D L Crawford
Journal:  Appl Environ Microbiol       Date:  1988-09       Impact factor: 4.792

3.  Esterase Activities of Fibrobacter succinogenes subsp. succinogenes S85.

Authors:  K P McDermid; C R Mackenzie; C W Forsberg
Journal:  Appl Environ Microbiol       Date:  1990-01       Impact factor: 4.792

4.  Induction of Cellulolytic and Xylanolytic Enzyme Systems in Streptomyces spp.

Authors:  C R Mackenzie; D Bilous; H Schneider; K G Johnson
Journal:  Appl Environ Microbiol       Date:  1987-12       Impact factor: 4.792

Review 5.  Esterases of xylan-degrading microorganisms: production, properties, and significance.

Authors:  L P Christov; B A Prior
Journal:  Enzyme Microb Technol       Date:  1993-06       Impact factor: 3.493

6.  Isolation and characterization of p-coumaroyl esterase from the anaerobic fungus Neocallimastix strain MC-2.

Authors:  W S Borneman; L G Ljungdahl; R D Hartley; D E Akin
Journal:  Appl Environ Microbiol       Date:  1991-08       Impact factor: 4.792

7.  Assay for trans-p-coumaroyl esterase using a specific substrate from plant cell walls.

Authors:  W S Borneman; R D Hartley; D S Himmelsbach; L G Ljungdahl
Journal:  Anal Biochem       Date:  1990-10       Impact factor: 3.365
  7 in total

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