| Literature DB >> 24415079 |
B K Rana1, B N Johri, I S Thakur.
Abstract
A thermophilic fungus, Humicola grisea var thermoidea, produced in liquid culture two endoxylanases (1,4-β-D-xylan-xylanohydrolase, EC 3.2.1.8) with M r of 95 (Xyl I) and 13 (Xyl II) kDa. PAGE of the crude culture filtrate and of each fraction obtained by gel filtration produced three and one band, respectively. Cross-reaction of the culture filtrate and each fraction with polyclonal antibodies prepared against Xyl II produced two and one precipitin bands, respectively. Hydrolysis of wheat straw and rice husk xylan was maximal using a combination of Xyl I and Xyl II. The products formed after hydrolysis, xylo-oligosaccharides and traces of xylose, indicated an endotype enzyme action and the co-operative activities of the xylanases.Entities:
Year: 1996 PMID: 24415079 DOI: 10.1007/BF00327791
Source DB: PubMed Journal: World J Microbiol Biotechnol ISSN: 0959-3993 Impact factor: 3.312