Studies of excitation energy transfer within the green alga Chlamydomonas reinhardtii and its mutants at 77 K.

The 77 K picosecond fluorescence of intact Chlamydomonas reinhardtii exhibits a 680-nm band (F680) that can be identified with light-harvesting chlorophyll. Analysis of the time and spectral dependence of F680 reveal a forward transfer rate of 1/(15 ps) from this 680-nm species to photosystem II. The possibility of transfer through LHC I, the light-harvesting complex closely associated with photosystem I with a transfer time of 60 to 100 ps, is indicated by analysis of similar data in the 700-720 nm region. Simple kinetic models that account for the time dependence of the emissions F707, F703 and F715 are proposed.