AIM: This study aims at reporting the isolation, structure elucidation, and antioxidant potentials of ajugoside from C. volubile leaves in sodium nitroprusside (SNP)-induced oxidative stressed rat brain and hepatic tissues. MATERIALS AND METHOD: An iridoid monoterpene, ajugoside was isolated from the n-butanol fraction of C. volubile and evaluated for its antioxidant protective potential on brain and liver tissues of male Wister rats in an ex vivo model. Two molar concentrations (6.4 × 10(-4) M and 1.28 × 10(-3) M) of the metabolite and SNP were incubated with the tissues homogenate at 37°C for 2 hr prior to the test and assayed for catalase, superoxide dismutase (SOD) activities, and lipid peroxidation. α tocopherol (6.4 × 10(-4) M) was used as standard. RESULTS: Both molar concentrations exhibited high catalase activity in the tissues. However, 6.4 × 10(-4) M ajugoside exhibited a very high SOD activity (liver: 96.45 and brain: 96.30%) and inhibition of lipid peroxidation (liver: 88.11 and brain: 93.27%) compared to the standard. 1.28 × 10(-3) M ajugoside also exhibited good activities but lower than that of the standard and 6.4 × 10(-4) M ajugoside. DISCUSSION AND CONCLUSION: Ajugoside showed potent antioxidant activities as evidenced by the synergistic high activities of SOD and catalase as well as inhibition of lipid peroxidation in the studied tissues.
AIM: This study aims at reporting the isolation, structure elucidation, and antioxidant potentials of ajugoside from C. volubile leaves in sodium nitroprusside (SNP)-induced oxidative stressed rat brain and hepatic tissues. MATERIALS AND METHOD: An iridoidmonoterpene, ajugoside was isolated from the n-butanol fraction of C. volubile and evaluated for its antioxidant protective potential on brain and liver tissues of male Wister rats in an ex vivo model. Two molar concentrations (6.4 × 10(-4) M and 1.28 × 10(-3) M) of the metabolite and SNP were incubated with the tissues homogenate at 37°C for 2 hr prior to the test and assayed for catalase, superoxide dismutase (SOD) activities, and lipid peroxidation. α tocopherol (6.4 × 10(-4) M) was used as standard. RESULTS: Both molar concentrations exhibited high catalase activity in the tissues. However, 6.4 × 10(-4) M ajugoside exhibited a very high SOD activity (liver: 96.45 and brain: 96.30%) and inhibition of lipid peroxidation (liver: 88.11 and brain: 93.27%) compared to the standard. 1.28 × 10(-3) M ajugoside also exhibited good activities but lower than that of the standard and 6.4 × 10(-4) M ajugoside. DISCUSSION AND CONCLUSION:Ajugoside showed potent antioxidant activities as evidenced by the synergistic high activities of SOD and catalase as well as inhibition of lipid peroxidation in the studied tissues.
Authors: Ochuko L Erukainure; Rahman M Hafizur; Nurul Kabir; M Iqbal Choudhary; Olubunmi Atolani; Priyanka Banerjee; Robert Preissner; Chika I Chukwuma; Aliyu Muhammad; Eric O Amonsou; Md Shahidul Islam Journal: Front Pharmacol Date: 2018-02-01 Impact factor: 5.810
Authors: Ochuko L Erukainure; Olajumoke A Oyebode; Veronica F Salau; Neil A Koorbanally; Md Shahidul Islam Journal: J Diabetes Metab Disord Date: 2019-11-18
Authors: Ochuko L Erukainure; Nadia Ashraf; Asma S Naqvi; Moses Z Zaruwa; Aliyu Muhammad; Adenike D Odusote; Gloria N Elemo Journal: Front Pharmacol Date: 2018-03-20 Impact factor: 5.810