The mitochondrial genome of the black dwarf honey bee, Apis andreniformis (Hymenoptera: Apidae).

We sequenced 17,329 bp of the black dwarf honey bee, Apis andreniformis (Hymenoptera: Apidae) mitochondrial genome (mitogenome) that lacked ∼200 bp of the A+T-rich region for the complete genomic sequence. The gene arrangement of the A. andreniformis mitogenome was identical to that of A. cerana. However, the genome contained five additional tRNALeu(CUN); four copies were located between tRNAMet and tRNAGln, and one copy was between tRNAGln and tRNAAla, along with the typical sets of genes (13 protein-coding genes, 22 tRNAs, and 2 rRNAs) including regular tRNALeu(CUN) and the A+T-rich region (at least 923 bp). Only one copy of tRNALeu(CUN) differed by 1 bp from the other four copies of tRNALeu(CUN). Each additional tRNALeu(CUN) was followed by a nearly identical 68-bp long repeat sequence (95.6% identity). All 13 protein coding genes had typica start codons found in insect mitochondrial protein coding genes (two ATA, nine ATT and two ATG).