The influence of gibberellic acid on reassociation kinetics of DNA of Daucus carota L.

Carrot DNA, extracted from the tap root of untreated and gibberellic acid (GA3)-treated plants and of different varieties, was analyzed by reassociation kinetics. Differences due to GA3 treatment appear mainly in the intermediate repeated DNA region. Differences in approximately the same region are found using carrot DNA of different varieties, which also show differences in the slow reassociating sequences. By hybridizing a "family" of the "unique" DNA range with DNA obtained from GA3-treated plants and the controls, respectively, it could be shown that changes in the composition of total DNA are the result of GA3 treatment.