Literature DB >> 24407993

Plant tRNA nucleotidyltransferase : I. Isolation and purification of tRNA nucleotidyltransferase from Lupinus luteus seeds.

H Cudny1, M Pietrzak, J Kaczkowski.   

Abstract

A method for purification of tRNA nucleotidyltransferase from Lupinus luteus L. seeds on a preparative scale is presented. This involved ammonium sulfate fractionation and chromatography on DEAE-cellulose, hydroxylapatite, and QAE-Sephadex A-50. The final enzyme prepartion was apparently homogeneous and a 2000-fold purification was achieved. Data presented suggest the existence of only one form of tRNA nucleotidyltransferase in dry lupin seeds.

Entities:  

Year:  1978        PMID： 24407993     DOI： 10.1007/BF00385115

Source DB:  PubMed          Journal:  Planta        ISSN： 0032-0935            Impact factor:   4.116


  9 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Changes of tRNA CCA end during germination of Lupinus luteus seeds.

Authors:  T Dziegielewski; J Pawelkiewicz
Journal:  Bull Acad Pol Sci Biol       Date:  1977

3.  Isolation and properties of tRNA nucleotidyltransferase from wheat embryos.

Authors:  P Dullin; A Fabisz-Kijowska; W Walerych
Journal:  Acta Biochim Pol       Date:  1975       Impact factor: 2.149

4.  Studies on tRNA nucleotidyltransferase from baker's yeast. 1. Purification of the enzyme. Protection against thermal inactivation and inhibition by several substrates.

Authors:  B Rether; J Bonnet; J P Ebel
Journal:  Eur J Biochem       Date:  1974-12-16

5.  Isolation and properties of tRNA nucleotidyl transferase from yeast.

Authors:  H Sternbach; F von der Haar; E Schlimme; E Gaertner; F Cramer
Journal:  Eur J Biochem       Date:  1971-09-24

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Purification and properties of Escherichia coli CTP (ATP)-tRNA nucleotidyltransferase.

Authors:  D S Carre; S Litvak; F Chapeville
Journal:  Biochim Biophys Acta       Date:  1970-12-14

8.  Transfer ribonucleic acid nucleotidyltransferase from Escherichia coli. II. Purification, physical properties, and substrate specificity.

Authors:  J P Miller; G R Philipps
Journal:  J Biol Chem       Date:  1971-03-10       Impact factor: 5.157

9.  Reactions at the 3' terminus of transfer ribonucleic acid. II. Purification and physical and chemical properties of rabbit liver transfer ribonucleic acid nucleotidyltransferase.

Authors:  M P Deutscher
Journal:  J Biol Chem       Date:  1972-01-25       Impact factor: 5.157
  9 in total
  2 in total

1.  Plant tRNA nucleotidyltransferase : II. Some properties of the purified enzyme from Lupinus luteus seeds.

Authors:  H Cudny; M Pietrzak; J Kaczkowski
Journal:  Planta       Date:  1978-01       Impact factor: 4.116

2.  Purification and characterization of a tRNA nucleotidyltransferase from Lupinus albus and functional complementation of a yeast mutation by corresponding cDNA.

Authors:  K Shanmugam; P J Hanic-Joyce; P B Joyce
Journal:  Plant Mol Biol       Date:  1996-01       Impact factor: 4.076
  2 in total

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