Miho Ogawa1, Kentaro Yamashita2, Minori Niikura3, Kei Nakajima4, Koh-ei Toyoshima5, Masamitsu Oshima5, Takashi Tsuji6. 1. Organ Technologies Inc., Chiyoda-ku, Tokyo 101-0048, Japan; Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan. 2. Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan. 3. Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan. 4. Department of Clinical Pathophysiology, Tokyo Dental College, Chiba-shi, Chiba 261-8502, Japan. 5. Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan. 6. Organ Technologies Inc., Chiyoda-ku, Tokyo 101-0048, Japan; Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan; Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan. Electronic address: t-tsuji@rs.noda.tus.ac.jp.
Abstract
PURPOSE: The aim of this study was to compare saliva flow and protein composition induced using five basic taste stimulations between natural and bioengineered salivary glands. MATERIALS AND METHODS: We developed a mouse saliva secretion model using taste stimulation and analyzed the saliva secretion from natural and bioengineered salivary glands using an assay. The protein components and alpha-amylase in the natural and bioengineered saliva were analyzed by gel electrophoresis and Western blotting. RESULTS: The salivary flow responses induced by sour (citric acid) and bitter (quinine-HCl) stimuli were significantly high in the natural and bioengineered salivary glands. Although the protein concentrations in the natural and bioengineered saliva induced using five basic taste stimulations were similar, the protein composition and the amylase concentration in the natural saliva after taste stimulation had different profiles. Sympathetic and non-sympathetic nerves were observed around the acini and ducts in the natural and bioengineered salivary glands. However, the frequency of neuropeptide Y-positive sympathetic nerves in the bioengineered gland was relatively high compared to that in the natural gland. CONCLUSIONS: These results suggest that the signal balance between the sympathetic and parasympathetic components of the efferent nerves in an engrafted bioengineered salivary gland may differ from that in a natural salivary gland.
PURPOSE: The aim of this study was to compare saliva flow and protein composition induced using five basic taste stimulations between natural and bioengineered salivary glands. MATERIALS AND METHODS: We developed a mouse saliva secretion model using taste stimulation and analyzed the saliva secretion from natural and bioengineered salivary glands using an assay. The protein components and alpha-amylase in the natural and bioengineered saliva were analyzed by gel electrophoresis and Western blotting. RESULTS: The salivary flow responses induced by sour (citric acid) and bitter (quinine-HCl) stimuli were significantly high in the natural and bioengineered salivary glands. Although the protein concentrations in the natural and bioengineered saliva induced using five basic taste stimulations were similar, the protein composition and the amylase concentration in the natural saliva after taste stimulation had different profiles. Sympathetic and non-sympathetic nerves were observed around the acini and ducts in the natural and bioengineered salivary glands. However, the frequency of neuropeptide Y-positive sympathetic nerves in the bioengineered gland was relatively high compared to that in the natural gland. CONCLUSIONS: These results suggest that the signal balance between the sympathetic and parasympathetic components of the efferent nerves in an engrafted bioengineered salivary gland may differ from that in a natural salivary gland.