Literature DB >> 2440346

Quantitative electrotransfer of proteins from sodium dodecyl sulfate-polyacrylamide gels onto positively charged nylon membranes.

R W Peluso, G H Rosenberg.   

Abstract

A method for the reproducible and quantitative electrotransfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a single sheet of either Zetabind or Gene Screen Plus membranes is presented. This procedure uses commercially available equipment and includes three crucial parameters: the omission of methanol from the transfer buffer, the use of thin (0.75-mm) resolving gels, and a newly developed protocol for pretreatment of the polyacrylamide gel after electrophoresis and before electroblotting. This combination of parameters yields a blot that both qualitatively and quantitatively reflects the proteins in the original polyacrylamide gel.

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Year:  1987        PMID： 2440346     DOI： 10.1016/0003-2697(87)90409-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  14 in total

1.  Localization and quantitation of chloroplast enzymes and light-harvesting components using immunocytochemical methods.

Authors:  L Mustardy; F X Cunningham; E Gantt
Journal:  Plant Physiol       Date:  1990-09       Impact factor: 8.340

2.  Decrease of polypeptides in the PS I antenna complex with increasing growth irradiance in the red alga Porphyridium cruentum.

Authors:  S Tan; G R Wolfe; F X Cunningham; E Gantt
Journal:  Photosynth Res       Date:  1995-07       Impact factor: 3.573

3.  Direct matrix-assisted laser desorption ionization mass spectrometric analysis of proteins immobilized on nylon-based membranes.

Authors:  E J Zaluzec; D A Gage; J Allison; J Throck Watson
Journal:  J Am Soc Mass Spectrom       Date:  1994-04       Impact factor: 3.109

4.  LhcaR1 of the red alga Porphyridium cruentum encodes a polypeptide of the LHCI complex with seven potential chlorophyll a-binding residues that are conserved in most LHCs.

Authors:  S Tan; F X Cunningham; E Gantt
Journal:  Plant Mol Biol       Date:  1997-01       Impact factor: 4.076

5.  Conserved motifs II to VI of DNA helicase II from Escherichia coli are all required for biological activity.

Authors:  G Zhang; E Deng; L R Baugh; C M Hamilton; V F Maples; S R Kushner
Journal:  J Bacteriol       Date:  1997-12       Impact factor: 3.490

6.  Identification and characterization of Escherichia coli DNA helicase II mutants that exhibit increased unwinding efficiency.

Authors:  G Zhang; E Deng; L Baugh; S R Kushner
Journal:  J Bacteriol       Date:  1998-01       Impact factor: 3.490

7.  Characterization of the Porphyridium cruentum Chl a-binding LHC by in vitro reconstitution: LHCaR1 binds 8 Chl a molecules and proportionately more carotenoids than CAB proteins.

Authors:  B Grabowski; S Tan; F X Cunningham; E Gantt
Journal:  Photosynth Res       Date:  2000       Impact factor: 3.573

8.  Kinetic, quantitative, and functional analysis of multiple forms of the vesicular stomatitis virus nucleocapsid protein in infected cells.

Authors:  R W Peluso
Journal:  J Virol       Date:  1988-08       Impact factor: 5.103

9.  Differential expression of two isopentenyl pyrophosphate isomerases and enhanced carotenoid accumulation in a unicellular chlorophyte.

Authors:  Z Sun; F X Cunningham; E Gantt
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-15       Impact factor: 11.205

10.  The nuclear matrix protein NMP-1 is the transcription factor YY1.

Authors:  B Guo; P R Odgren; A J van Wijnen; T J Last; J Nickerson; S Penman; J B Lian; J L Stein; G S Stein
Journal:  Proc Natl Acad Sci U S A       Date:  1995-11-07       Impact factor: 11.205
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