G I Marino1, B A Kotsias2. 1. Laboratorio de Canales Iónicos, Instituto de Investigaciones Médicas A. Lanari, IDIM-CONICET, Universidad de Buenos Aires, C. de Malvinas 3150, 1427 Buenos Aires, Argentina. Electronic address: gabinemar@gmail.com. 2. Laboratorio de Canales Iónicos, Instituto de Investigaciones Médicas A. Lanari, IDIM-CONICET, Universidad de Buenos Aires, C. de Malvinas 3150, 1427 Buenos Aires, Argentina.
Abstract
INTRODUCTION: ENaC and CFTR are coexpressed in epithelia and have positive or negative functional interactions. In addition, ENaC and CFTR promote migration in placental trophoblastic cells and human airway cells, respectively. Here we tested the idea if CFTR is functionally expressed in BeWo cells, a trophoblastic cell line, and if it is involved in their migratory behavior. METHODS: CFTR expression was studied in BeWo cells with RT-PCR, biotinylation and Western blot. Ion currents were analyzed with patch clamp, and cell migration with the wound healing method. RESULTS: The mature CFTR 160-kDa band was present, and its localization at the surface membrane was confirmed. Forskolin (20 μM), an adenylate cyclase activator, was used for channel activation, and subsequently CFTR(inh)-172 (2 μM) for its inhibition. The conductances in the presence of CFTR(inh)-172 plus forskolin (16.0 ± 0.7 pS/pF and 32.6 ± 1.5 pS/pF) were significantly lower than in presence of only forskolin (29.7 ± 0.9 and 47.0 ± 2.0 pS/pF). The conductance of CFTR(inh)-172 inhibited currents was 14.9 ± 0.7 pS/pF with a linear I-V relationship illustrating the nonrectifying properties of the CFTR. Cell migration was measured and covered 11.2 ± 0.4, 24.0 ± 1.7 and 13.9 ± 1.0% of the wound when cells were cultivated under control, forskolin, and forskolin plus CFTR(inh)-172, respectively. Proliferation was not changed by any of the treatments. CONCLUSIONS: Our results shows that BeWo cells functionally express the CFTR which plays a role in the wound healing increasing the cell migration process.
INTRODUCTION: ENaC and CFTR are coexpressed in epithelia and have positive or negative functional interactions. In addition, ENaC and CFTR promote migration in placental trophoblastic cells and human airway cells, respectively. Here we tested the idea if CFTR is functionally expressed in BeWo cells, a trophoblastic cell line, and if it is involved in their migratory behavior. METHODS:CFTR expression was studied in BeWo cells with RT-PCR, biotinylation and Western blot. Ion currents were analyzed with patch clamp, and cell migration with the wound healing method. RESULTS: The mature CFTR 160-kDa band was present, and its localization at the surface membrane was confirmed. Forskolin (20 μM), an adenylate cyclase activator, was used for channel activation, and subsequently CFTR(inh)-172 (2 μM) for its inhibition. The conductances in the presence of CFTR(inh)-172 plus forskolin (16.0 ± 0.7 pS/pF and 32.6 ± 1.5 pS/pF) were significantly lower than in presence of only forskolin (29.7 ± 0.9 and 47.0 ± 2.0 pS/pF). The conductance of CFTR(inh)-172 inhibited currents was 14.9 ± 0.7 pS/pF with a linear I-V relationship illustrating the nonrectifying properties of the CFTR. Cell migration was measured and covered 11.2 ± 0.4, 24.0 ± 1.7 and 13.9 ± 1.0% of the wound when cells were cultivated under control, forskolin, and forskolin plus CFTR(inh)-172, respectively. Proliferation was not changed by any of the treatments. CONCLUSIONS: Our results shows that BeWo cells functionally express the CFTR which plays a role in the wound healing increasing the cell migration process.
Authors: Andreas Schwingshackl; Bin Teng; Patrudu Makena; Manik Ghosh; Scott E Sinclair; Charlean Luellen; Louisa Balasz; Cynthia Rovnaghi; Robert M Bryan; Eric E Lloyd; Elizabeth Fitzpatrick; Jordy S Saravia; Stephania A Cormier; Christopher M Waters Journal: Crit Care Med Date: 2014-11 Impact factor: 7.598
Authors: Andreas Schwingshackl; Benjamin Lopez; Bin Teng; Charlean Luellen; Florian Lesage; John Belperio; Riccardo Olcese; Christopher M Waters Journal: Am J Physiol Lung Cell Mol Physiol Date: 2017-08-24 Impact factor: 5.464