| Literature DB >> 24395362 |
Alejandro Sarrion-Perdigones1, Jorge Palaci, Antonio Granell, Diego Orzaez.
Abstract
GoldenBraid (GB) is an iterative and standardized DNA assembling system specially designed for Multigene Engineering in Plant Synthetic Biology. GB is based on restriction-ligation reactions using type IIS restriction enzymes. GB comprises a collection of standard DNA pieces named "GB parts" and a set of destination plasmids (pDGBs) that incorporate the multipartite assembly of standardized DNA parts. GB reactions are extremely efficient: two transcriptional units (TUs) can be assembled from several basic GBparts in one T-DNA less than 24 h. Moreover, larger assemblies comprising 4-5 TUs are routinely built in less than 2 working weeks. Here we provide a detailed view of the GB methodology. As a practical example, a Bimolecular Fluorescence Complementation construct comprising four TUs in a 12 kb DNA fragment is presented.Entities:
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Year: 2014 PMID： 24395362 DOI： 10.1007/978-1-62703-764-8_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745