Literature DB >> 24388586

Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus.

Chee-Keng Mok1, Shih-Cheng Chang2, Guang-Wu Chen3, Yu-Lun Lo2, Shu-Jen Chen4, Ho-Sheng Wu5, Ming-Tsan Liu5, Feng-Yee Chang5, Tzou-Yien Lin6, Shin-Ru Shih7.   

Abstract

Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.
Copyright © 2013. Published by Elsevier B.V.

Entities:  

Keywords:  Avian influenza A virus; H7N9; Oseltamivir-resistant marker; Pyrosequencing

Mesh：

Substances：

Year:  2013        PMID： 24388586     DOI： 10.1016/j.jmii.2013.09.010

Source DB:  PubMed          Journal:  J Microbiol Immunol Infect        ISSN： 1684-1182            Impact factor:   4.399


  7 in total

1.  Characterization of drug-resistant influenza A(H7N9) variants isolated from an oseltamivir-treated patient in Taiwan.

Authors:  Henju Marjuki; Vasiliy P Mishin; Anton P Chesnokov; Joyce Jones; Juan A De La Cruz; Katrina Sleeman; Daisuke Tamura; Ha T Nguyen; Ho-Sheng Wu; Feng-Yee Chang; Ming-Tsan Liu; Alicia M Fry; Nancy J Cox; Julie M Villanueva; Charles T Davis; Larisa V Gubareva
Journal:  J Infect Dis       Date:  2014-08-14       Impact factor: 5.226

Review 2.  Deep sequencing: becoming a critical tool in clinical virology.

Authors:  Miguel E Quiñones-Mateu; Santiago Avila; Gustavo Reyes-Teran; Miguel A Martinez
Journal:  J Clin Virol       Date:  2014-06-24       Impact factor: 3.168

3.  Genomic Signatures for Avian H7N9 Viruses Adapting to Humans.

Authors:  Guang-Wu Chen; Shu-Ming Kuo; Shu-Li Yang; Yu-Nong Gong; Mei-Ren Hsiao; Yi-Chun Liu; Shin-Ru Shih; Kuo-Chien Tsao
Journal:  PLoS One       Date:  2016-02-04       Impact factor: 3.240

4.  The K526R substitution in viral protein PB2 enhances the effects of E627K on influenza virus replication.

Authors:  Wenjun Song; Pui Wang; Bobo Wing-Yee Mok; Siu-Ying Lau; Xiaofeng Huang; Wai-Lan Wu; Min Zheng; Xi Wen; Shigui Yang; Yu Chen; Lanjuan Li; Kwok-Yung Yuen; Honglin Chen
Journal:  Nat Commun       Date:  2014-11-20       Impact factor: 14.919

5.  A Next-Generation Sequencing Data Analysis Pipeline for Detecting Unknown Pathogens from Mixed Clinical Samples and Revealing Their Genetic Diversity.

Authors:  Yu-Nong Gong; Guang-Wu Chen; Shu-Li Yang; Ching-Ju Lee; Shin-Ru Shih; Kuo-Chien Tsao
Journal:  PLoS One       Date:  2016-03-17       Impact factor: 3.240

6.  Virus-induced pathogenesis, vaccine development, and diagnosis of novel H7N9 avian influenza A virus in humans: a systemic literature review.

Authors:  Wen-Hung Wang; Esmeralda Merari Erazo; Max R Chang Ishcol; Chih-Yen Lin; Wanchai Assavalapsakul; Arunee Thitithanyanont; Sheng-Fan Wang
Journal:  J Int Med Res       Date:  2019-05-08       Impact factor: 1.671

7.  Phenotypic and Genetic Characterization of Avian Influenza H5N2 Viruses with Intra- and Inter-Duck Variations in Taiwan.

Authors:  Yao-Tsun Li; Hui-Ying Ko; Chang-Chun David Lee; Ching-Yu Lai; Chuan-Liang Kao; Chinglai Yang; Won-Bo Wang; Chwan-Chuen King
Journal:  PLoS One       Date:  2015-08-11       Impact factor: 3.240
  7 in total

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