Literature DB >> 2438655

Site-directed cleavage of RNA.

S Shibahara, S Mukai, T Nishihara, H Inoue, E Ohtsuka, H Morisawa.   

Abstract

Using complementary chimeric oligonucleotides containing deoxyribonucleotides and 2'-O-methylribonucleotides (1), enzymatically synthesized RNA (90 mer) were cleaved at a single site with Escherichia coli RNaseH, either at a hairpin loop or at a stem region. Especially, site-specific cleavage occurred in even the target region being enclosed within a stable, base-paired stem. The method is proved to be generally applicable to RNA containing secondary structures.

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Year:  1987        PMID： 2438655      PMCID： PMC340870          DOI： 10.1093/nar/15.11.4403

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  17 in total

1.  Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA.

Authors:  K L Berkner; W R Folk
Journal:  J Biol Chem       Date:  1977-05-25       Impact factor: 5.157

2.  New rapid gel sequencing method for RNA.

Authors:  A Simoncsits; G G Brownlee; R S Brown; J R Rubin; H Guilley
Journal:  Nature       Date:  1977-10-27       Impact factor: 49.962

3.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

4.  A hydridase from Escherichia coli.

Authors:  C M Henry; F J Ferdinand; R Knippers
Journal:  Biochem Biophys Res Commun       Date:  1973-02-05       Impact factor: 3.575

5.  Isolation and characterization of an endonuclease from Escherichia coli specific for ribonucleic acid in ribonucleic acid-deoxyribonucleic acid hybrid structures.

Authors:  I Berkower; J Leis; J Hurwitz
Journal:  J Biol Chem       Date:  1973-09-10       Impact factor: 5.157

6.  Nucleic acid related compounds. 12. The facile and high-yield stannous chloride catalyzed monomethylation of the cis-glycol system of nucleosides by diazomethane.

Authors:  M J Robins; S R Naik; A S Lee
Journal:  J Org Chem       Date:  1974-06-28       Impact factor: 4.354

7.  Ribonuclease H. An enzyme degrading the RNA moiety of DNA-RNA hybrids.

Authors:  P Hausen; H Stein
Journal:  Eur J Biochem       Date:  1970-06

8.  Enzyme from calf thymus degrading the RNA moiety of DNA-RNA Hybrids: effect on DNA-dependent RNA polymerase.

Authors:  H Stein; P Hausen
Journal:  Science       Date:  1969-10-17       Impact factor: 47.728

9.  3'-terminal labelling of RNA with T4 RNA ligase.

Authors:  T E England; O C Uhlenbeck
Journal:  Nature       Date:  1978-10-12       Impact factor: 49.962

10.  Mapping adenines, guanines, and pyrimidines in RNA.

Authors:  H Donis-Keller; A M Maxam; W Gilbert
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971
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  26 in total

1.  A simple procedure for the preparation of protected 2'-O-methyl or 2'-O-ethyl ribonucleoside-3'-O-phosphoramidites.

Authors:  E Wagner; B Oberhauser; A Holzner; H Brunar; G Issakides; G Schaffner; M Cotten; M Knollmüller; C R Noe
Journal:  Nucleic Acids Res       Date:  1991-11-11       Impact factor: 16.971

2.  A new technique for the characterization of long-range tertiary contacts in large RNA molecules: insertion of a photolabel at a selected position in 16S rRNA within the Escherichia coli ribosome.

Authors:  P V Baranov; S S Dokudovskaya; T S Oretskaya; O A Dontsova; A A Bogdanov; R Brimacombe
Journal:  Nucleic Acids Res       Date:  1997-06-15       Impact factor: 16.971

3.  Deoxynucleotide-containing oligoribonucleotide duplexes: stability and susceptibility to RNase V1 and RNase H.

Authors:  J R Wyatt; G T Walker
Journal:  Nucleic Acids Res       Date:  1989-10-11       Impact factor: 16.971

4.  In vitro synthesis of infectious RNAs from cDNAs of cucumber mosaic virus satellite RNA (strain Y) after removal of non-viral bases with ribonuclease H.

Authors:  C Masuta; S Kuwata; Y Takanami
Journal:  Nucleic Acids Res       Date:  1987-12-10       Impact factor: 16.971

5.  Effects of oligo sequence and chemistry on the efficiency of oligodeoxyribonucleotide-mediated mRNA cleavage.

Authors:  C Baker; D Holland; M Edge; A Colman
Journal:  Nucleic Acids Res       Date:  1990-06-25       Impact factor: 16.971

6.  Design of RNA enzymes distinguishing a single base mutation in RNA.

Authors:  M Koizumi; Y Hayase; S Iwai; H Kamiya; H Inoue; E Ohtsuka
Journal:  Nucleic Acids Res       Date:  1989-09-12       Impact factor: 16.971

7.  Site-selective cleavage of structured RNA by a staphylococcal nuclease-DNA hybrid.

Authors:  R N Zuckermann; P G Schultz
Journal:  Proc Natl Acad Sci U S A       Date:  1989-03       Impact factor: 11.205

8.  Inhibition of human immunodeficiency virus (HIV-1) replication by synthetic oligo-RNA derivatives.

Authors:  S Shibahara; S Mukai; H Morisawa; H Nakashima; S Kobayashi; N Yamamoto
Journal:  Nucleic Acids Res       Date:  1989-01-11       Impact factor: 16.971

9.  Highly efficient chemical synthesis of 2'-O-methyloligoribonucleotides and tetrabiotinylated derivatives; novel probes that are resistant to degradation by RNA or DNA specific nucleases.

Authors:  B S Sproat; A I Lamond; B Beijer; P Neuner; U Ryder
Journal:  Nucleic Acids Res       Date:  1989-05-11       Impact factor: 16.971

10.  An improved synthesis of oligodeoxynucleotide N3'-->P5' phosphoramidates and their chimera using hindered phosphoramidite monomers and a novel handle for reverse phase purification.

Authors:  K L Fearon; B L Hirschbein; J S Nelson; M F Foy; M Q Nguyen; A Okruszek; S N McCurdy; J E Frediani; L A DeDionisio; A M Raible; E N Cagle; V Boyd
Journal:  Nucleic Acids Res       Date:  1998-08-15       Impact factor: 16.971
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