BACKGROUND AND OBJECTIVES: Salmonella typhi, Mycobacterium tuberculosis, and Burkholderia pseudomallei are among the most important monocyte-tropic bacterial agents causing pyrexia of unknown origin (PUO), with a significant number of endemic infections in both South and Southeast Asian regions. These infections pose a major risk to travelers to these regions as well. METHODS: We developed and evaluated a multiplex nested polymerase chain reaction (PCR) for the simultaneous detection of the three pathogens in 305 patients' buffy coat samples. RESULTS: The assay for S. typhi and B. pseudomallei was able to detect down to 1 colony forming unit/5 μL PCR input and M. tuberculosis was detected down to 20 genome copies/5 μL PCR input. S. typhi was detected in 10 (3.3 %) individuals, B. pseudomallei in 10 individuals (3.3 %), and M. tuberculosis in 18 individuals (5.9 %). Co-infections of M. tuberculosis and B. pseudomallei were detected in three individuals and S. typhi and B. pseudomallei in two individuals. CONCLUSION: This protocol is efficient for PUO diagnosis especially in Asian countries.
BACKGROUND AND OBJECTIVES:Salmonella typhi, Mycobacterium tuberculosis, and Burkholderia pseudomallei are among the most important monocyte-tropic bacterial agents causing pyrexia of unknown origin (PUO), with a significant number of endemic infections in both South and Southeast Asian regions. These infections pose a major risk to travelers to these regions as well. METHODS: We developed and evaluated a multiplex nested polymerase chain reaction (PCR) for the simultaneous detection of the three pathogens in 305 patients' buffy coat samples. RESULTS: The assay for S. typhi and B. pseudomallei was able to detect down to 1 colony forming unit/5 μL PCR input and M. tuberculosis was detected down to 20 genome copies/5 μL PCR input. S. typhi was detected in 10 (3.3 %) individuals, B. pseudomallei in 10 individuals (3.3 %), and M. tuberculosis in 18 individuals (5.9 %). Co-infections of M. tuberculosis and B. pseudomallei were detected in three individuals and S. typhi and B. pseudomallei in two individuals. CONCLUSION: This protocol is efficient for PUO diagnosis especially in Asian countries.
Authors: B Nandagopal; S Sankar; K Lingesan; K C Appu; G Sridharan; A K Gopinathan Journal: Indian J Med Microbiol Date: 2010 Jul-Sep Impact factor: 0.985
Authors: T Dharakul; S Songsivilai; S Viriyachitra; V Luangwedchakarn; B Tassaneetritap; W Chaowagul Journal: J Clin Microbiol Date: 1996-03 Impact factor: 5.948
Authors: J Wain; V B Pham; V Ha; N M Nguyen; S D To; A L Walsh; C M Parry; R P Hasserjian; V A HoHo; T H Tran; J Farrar; N J White; N P Day Journal: J Clin Microbiol Date: 2001-04 Impact factor: 5.948
Authors: John Wain; To Song Diep; Phan Van Be Bay; Amanda L Walsh; Ha Vinh; Nguyen M Duong; Vo Anh Ho; Tran T Hien; Jeremy Farrar; Nicholas J White; Christopher M Parry; Nicholas P J Day Journal: J Infect Dev Ctries Date: 2008-12-01 Impact factor: 0.968
Authors: Erika R Vlieghe; Thong Phe; Birgit De Smet; Heng Chhun Veng; Chun Kham; Kruy Lim; Olivier Koole; Lut Lynen; Willy E Peetermans; Jan A Jacobs Journal: PLoS One Date: 2013-03-29 Impact factor: 3.240