Erdim Sertoglu1, Ismail Kurt2, Serkan Tapan2, Metin Uyanik2, Muhittin A Serdar3, Huseyin Kayadibi4, Saad El-Fawaeir2. 1. Ankara Mevki Military Hospital, Anittepe Dispensary, Ankara, Turkey. Electronic address: erdimsertoglu@gmail.com. 2. Gulhane School of Medicine, Department of Medical Biochemistry, Ankara, Turkey. 3. Acıbadem University School of Medicine, Department of Medical Biochemistry, Istanbul, Turkey. 4. Adana Military Hospital, Department of Medical Biochemistry, Adana, Turkey.
Abstract
BACKGROUND: In this study, we aimed to compare the serum lipid profile and fatty acid (FA) compositions of erythrocyte membrane (EM) and plasma in three different patient groups (group 1: type 2 diabetes mellitus (T2DM)+end-stage renal disease (ESRD), group 2: ESRD, group 4: T2DM) and healthy controls (group 3) simultaneously. METHODS: 40 ESRD patients treated with hemodialysis (HD) in Gulhane School of Medicine (20 with T2DM) and 32 controls (17 with T2DM, 15 healthy controls) were included in the study. Plasma and EM FA concentrations were measured by gas chromatography-flame ionization detector (GC-FID). RESULTS: Plasma and EM palmitic acid (PA) and stearic acid (SA) levels were significantly higher in T2DM patients compared to controls (p=0.040 and p=0.002 for plasma, p=0.001 and p=0.010 for EM, respectively). EM docosahexaenoic acid (DHA) levels were also significantly lower in patients with ESRD+T2DM and ESRD compared to controls (p=0.004 and p=0.037, respectively). CONCLUSIONS: Patients with insulin resistance display a pattern of high long chain saturated FAs (PA, SA and arachidic acids). However, while there are no recognized standards for normal EM DHA content, decreased levels of EM DHA in ESRD patient groups (groups 1 and 2) suggest that there may be reduced endogenous synthesis of DHA in HD subjects, due to the decreased functionality of desaturase and elongase enzymes. Because membrane PUFA content affects membrane fluidity and cell signaling, these findings are worthy of further investigation.
BACKGROUND: In this study, we aimed to compare the serum lipid profile and fatty acid (FA) compositions of erythrocyte membrane (EM) and plasma in three different patient groups (group 1: type 2 diabetes mellitus (T2DM)+end-stage renal disease (ESRD), group 2: ESRD, group 4: T2DM) and healthy controls (group 3) simultaneously. METHODS: 40 ESRDpatients treated with hemodialysis (HD) in Gulhane School of Medicine (20 with T2DM) and 32 controls (17 with T2DM, 15 healthy controls) were included in the study. Plasma and EM FA concentrations were measured by gas chromatography-flame ionization detector (GC-FID). RESULTS: Plasma and EM palmitic acid (PA) and stearic acid (SA) levels were significantly higher in T2DM patients compared to controls (p=0.040 and p=0.002 for plasma, p=0.001 and p=0.010 for EM, respectively). EM docosahexaenoic acid (DHA) levels were also significantly lower in patients with ESRD+T2DM and ESRD compared to controls (p=0.004 and p=0.037, respectively). CONCLUSIONS:Patients with insulin resistance display a pattern of high long chain saturated FAs (PA, SA and arachidic acids). However, while there are no recognized standards for normal EM DHA content, decreased levels of EM DHA in ESRDpatient groups (groups 1 and 2) suggest that there may be reduced endogenous synthesis of DHA in HD subjects, due to the decreased functionality of desaturase and elongase enzymes. Because membrane PUFA content affects membrane fluidity and cell signaling, these findings are worthy of further investigation.
Authors: David J Beale; Farhana R Pinu; Konstantinos A Kouremenos; Mahesha M Poojary; Vinod K Narayana; Berin A Boughton; Komal Kanojia; Saravanan Dayalan; Oliver A H Jones; Daniel A Dias Journal: Metabolomics Date: 2018-11-17 Impact factor: 4.290
Authors: Lauren Boland; Anthony J Burand; Alex J Brown; Devlin Boyt; Vitor A Lira; James A Ankrum Journal: Mol Ther Date: 2017-12-19 Impact factor: 11.454