OBJECTIVE: This study assessed the relationship between the p53-dependent apoptosis and recurrent spontaneous abortion (RSA). PATIENTS AND METHODS: Thirty women with recurring miscarriages were enrolled as experimental group, and 30 women with normal reproduction served as control group. Immunohistochemistry was used to evaluate expression of p53 in villous tissue specimens. Further, expressions of CDKN1A and Bax mRNAs were evaluated by qPCR. TUNEL assay was utilized to document cell apoptosis. RESULTS: Expression of p53 was significantly increased in chorionic villi of patients in experimental group (p < 0.05 vs. control group). Further, CDKN1A and Bax mRNA levels were elevated in experimental group (p < 0.05 vs. control group), and the cell apoptosis index was increased as well. CONCLUSIONS: The p53-CDKN1A and p53-Bax signaling pathways appear to be activated in RSA. Thus, the apoptosis pathways controlled by p.
OBJECTIVE: This study assessed the relationship between the p53-dependent apoptosis and recurrent spontaneous abortion (RSA). PATIENTS AND METHODS: Thirty women with recurring miscarriages were enrolled as experimental group, and 30 women with normal reproduction served as control group. Immunohistochemistry was used to evaluate expression of p53 in villous tissue specimens. Further, expressions of CDKN1A and Bax mRNAs were evaluated by qPCR. TUNEL assay was utilized to document cell apoptosis. RESULTS: Expression of p53 was significantly increased in chorionic villi of patients in experimental group (p < 0.05 vs. control group). Further, CDKN1A and Bax mRNA levels were elevated in experimental group (p < 0.05 vs. control group), and the cell apoptosis index was increased as well. CONCLUSIONS: The p53-CDKN1A and p53-Bax signaling pathways appear to be activated in RSA. Thus, the apoptosis pathways controlled by p.
Authors: Rafael Tomoya Michita; Francis Maria Báo Zambra; Lucas Rosa Fraga; Maria Teresa Sanseverino; Lavínia Schuler-Faccini; José Artur Bogo Chies; Priscila Vianna Journal: J Assist Reprod Genet Date: 2019-04-02 Impact factor: 3.412
Authors: Yao Ye; Aurelia Vattai; Nina Ditsch; Christina Kuhn; Martina Rahmeh; Sven Mahner; Myriam Ripphahn; Roland Immler; Markus Sperandio; Udo Jeschke; Viktoria von Schönfeldt Journal: Endocr Connect Date: 2018-04-26 Impact factor: 3.335
Authors: Gary P Brennan; Dimitrios M Vitsios; Sophie Casey; Ann-Marie Looney; Boubou Hallberg; David C Henshall; Geraldine B Boylan; Deirdre M Murray; Catherine Mooney Journal: PLoS One Date: 2018-12-03 Impact factor: 3.240