BACKGROUND: The neonatal surgical patient is threatened by exuberant inflammatory reactions. Neonatal macrophages are key players in this process. We investigated the ability of neonatal macrophages to initiate a local inflammatory reaction upon exposure to different bacterial or viral ligands to toll-like receptors (TLRs). METHODS: Peritoneal wash outs from neonatal (<24 h) and adult (42 days) C57BL/6J mice were gained by peritoneal lavages. In a first set of experiments, macrophages were purified and stimulated for 6 h by four different TLR ligands. mRNA was extracted for transcriptome analysis. In a second set of experiments, lipopolysaccharide was applied into peritoneal cavities. After 6 h of incubation, the cellular composition of the inflamed cavities was evaluated by cytological staining as well as chipcytometry. RESULTS: Neonatal murine peritoneal macrophages differed significantly in the expression of pro- and anti-chemotactic genes. Functional assignment of these genes revealed enhanced chemotactic potential of neonatal macrophages and was confirmed by a higher influx of pro-inflammatory cells into neonatal peritoneal cavities. CONCLUSION: Neonatal peritoneal macrophages demonstrated an enhanced chemotactic potential upon stimulation with four TLR ligands. This was associated with an increased influx of inflammatory cells to the peritoneal cavity. This might contribute to the strong inflammatory responses of neonates and preterms.
BACKGROUND: The neonatal surgical patient is threatened by exuberant inflammatory reactions. Neonatal macrophages are key players in this process. We investigated the ability of neonatal macrophages to initiate a local inflammatory reaction upon exposure to different bacterial or viral ligands to toll-like receptors (TLRs). METHODS: Peritoneal wash outs from neonatal (<24 h) and adult (42 days) C57BL/6J mice were gained by peritoneal lavages. In a first set of experiments, macrophages were purified and stimulated for 6 h by four different TLR ligands. mRNA was extracted for transcriptome analysis. In a second set of experiments, lipopolysaccharide was applied into peritoneal cavities. After 6 h of incubation, the cellular composition of the inflamed cavities was evaluated by cytological staining as well as chipcytometry. RESULTS: Neonatal murine peritoneal macrophages differed significantly in the expression of pro- and anti-chemotactic genes. Functional assignment of these genes revealed enhanced chemotactic potential of neonatal macrophages and was confirmed by a higher influx of pro-inflammatory cells into neonatal peritoneal cavities. CONCLUSION: Neonatal peritoneal macrophages demonstrated an enhanced chemotactic potential upon stimulation with four TLR ligands. This was associated with an increased influx of inflammatory cells to the peritoneal cavity. This might contribute to the strong inflammatory responses of neonates and preterms.
Authors: Amin Afrazi; Chhinder P Sodhi; Ward Richardson; Matthew Neal; Misty Good; Richard Siggers; David J Hackam Journal: Pediatr Res Date: 2011-03 Impact factor: 3.756
Authors: Ofer Levy; Kol A Zarember; Rene M Roy; Colette Cywes; Paul J Godowski; Michael R Wessels Journal: J Immunol Date: 2004-10-01 Impact factor: 5.422
Authors: Daigo Hashimoto; Andrew Chow; Clara Noizat; Pearline Teo; Mary Beth Beasley; Marylene Leboeuf; Christian D Becker; Peter See; Jeremy Price; Daniel Lucas; Melanie Greter; Arthur Mortha; Scott W Boyer; E Camilla Forsberg; Masato Tanaka; Nico van Rooijen; Adolfo García-Sastre; E Richard Stanley; Florent Ginhoux; Paul S Frenette; Miriam Merad Journal: Immunity Date: 2013-04-18 Impact factor: 31.745
Authors: Nathan P Corbett; Darren Blimkie; Kevin C Ho; Bing Cai; Darren P Sutherland; Arlene Kallos; Juliet Crabtree; Annie Rein-Weston; Pascal M Lavoie; Stuart E Turvey; Natalie R Hawkins; Steven G Self; Christopher B Wilson; Adeline M Hajjar; Edgardo S Fortuno; Tobias R Kollmann Journal: PLoS One Date: 2010-11-30 Impact factor: 3.240