Literature DB >> 2437824

A rapid method for staining proteins in acrylamide gels.

G A LeBlanc, B J Cochrane.   

Abstract

A negative staining procedure for the rapid visualization of proteins in acrylamide gels is described. In the absence of proteins, staining of the gel occurs through the reduction of nitroblue tetrazolium by reduced glutathione. No staining occurs in the presence of proteins. The procedure can be completed in 20 min and is at least as sensitive as Coomassie brilliant blue staining.

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Year:  1987        PMID： 2437824     DOI： 10.1016/0003-2697(87)90668-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  Quantitative proteomics: assessing the spectrum of in-gel protein detection methods.

Authors:  Victoria J Gauci; Elise P Wright; Jens R Coorssen
Journal:  J Chem Biol       Date:  2010-06-19

2.  Reversible and irreversible cross-linking of immunoglobulin heavy chains through their carbohydrate residues.

Authors:  U Heimgartner; B Kozulić; K Mosbach
Journal:  Biochem J       Date:  1990-05-01       Impact factor: 3.857

3.  Glycoproteins composed of major surface immunodeterminants of Pneumocystis carinii.

Authors:  K Tanabe; S Takasaki; J Watanabe; A Kobata; K Egawa; Y Nakamura
Journal:  Infect Immun       Date:  1989-05       Impact factor: 3.441
  3 in total

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