Jun-yi Luo1, Yi-tong Ma2, Xiang Xie1, Yi-ning Yang1, Xiang Ma1, Zhen-yan Fu1, Xiao-mei Li1, Zi-xiang Yu1, Bang-dang Chen3, Fen Liu3. 1. Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054, China. 2. Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054, China. Email: myt-xj@163.com. 3. Xinjiang Key Laboratory of Cardiovascular Disease Research.
Abstract
OBJECTIVE: To explore the distribution on K469E single nucleotide polymorphism of ICAM-1 gene among people with Uygur ethnicity, in Xinjiang and to analyze the correlation between ICAM-1 gene polymorphism and coronary heart disease. METHODS: 245 patients with coronary heart disease patients and 377 healthy controls in Xinjiang Uygur population were studied. ICAM-1 gene K469E genotype located in exon 6 were detected by polymerase chain reaction-restricted fragments length polymorphism. RESULTS: Distribution of genotypes in the two groups appeared to be in Hardy-Weinberg equilibrium (P > 0.05). The distribution of genotypes showed significant difference between the two groups (P = 0.039)and the distributions of K and E allele also presented statistically significant difference (P = 0.031). Significant difference was also observed in males(P = 0.029 for genotype, P = 0.025 for allele)but not in females. After adjusted for confounding factors, results from logistic regression analysis indicated that KK genotype was a risk factor for CHD in Uygur male population (OR = 2.389, 95% CI:1.458-3.915, P = 0.001). CONCLUSION: Genetic polymorphism of ICAM-1 K469E might increase the risk for coronary artery disease in males of Uygur patients in Xinjiang.
OBJECTIVE: To explore the distribution on K469E single nucleotide polymorphism of ICAM-1 gene among people with Uygur ethnicity, in Xinjiang and to analyze the correlation between ICAM-1 gene polymorphism and coronary heart disease. METHODS: 245 patients with coronary heart diseasepatients and 377 healthy controls in Xinjiang Uygur population were studied. ICAM-1 gene K469E genotype located in exon 6 were detected by polymerase chain reaction-restricted fragments length polymorphism. RESULTS: Distribution of genotypes in the two groups appeared to be in Hardy-Weinberg equilibrium (P > 0.05). The distribution of genotypes showed significant difference between the two groups (P = 0.039)and the distributions of K and E allele also presented statistically significant difference (P = 0.031). Significant difference was also observed in males(P = 0.029 for genotype, P = 0.025 for allele)but not in females. After adjusted for confounding factors, results from logistic regression analysis indicated that KK genotype was a risk factor for CHD in Uygur male population (OR = 2.389, 95% CI:1.458-3.915, P = 0.001). CONCLUSION: Genetic polymorphism of ICAM-1K469E might increase the risk for coronary artery disease in males of Uygur patients in Xinjiang.