BACKGROUND: TIAM2, a Rac guanine nucleotide exchange factor, is closely associated with cell adherence and migration. Here, we aimed to investigate the role of TIAM2 in non-small cell lung cancer (NSCLC) cells. MATERIALS AND METHODS: A small interference RNA (siRNA) was introduced to silence the expression of TIAM2. Invasion and motility assays were then performed to assess the invasion and motility potential of NSCLC cells. GST-pull down assays were used to detect activation of Rac1. RESULTS: TIAM2 was highly expressed in NSCLC cells. Knockdown of TIAM2 inhibited the invasion and motility, and suppressed activation of Rac1. Further experiments demonstrated that knockdown of TIAM2 could up-regulate the expression of E-cadherin, and down- regulate the expression of MMP-3, Twist and Snail. CONCLUSIONS: Our data suggest that TIAM2 can promote invasion and motility of NSCLC cells. Activation of Rac1 and regulation of some EMT/invasion-related genes may be involved in the underlying processes.
BACKGROUND:TIAM2, a Rac guanine nucleotide exchange factor, is closely associated with cell adherence and migration. Here, we aimed to investigate the role of TIAM2 in non-small cell lung cancer (NSCLC) cells. MATERIALS AND METHODS: A small interference RNA (siRNA) was introduced to silence the expression of TIAM2. Invasion and motility assays were then performed to assess the invasion and motility potential of NSCLC cells. GST-pull down assays were used to detect activation of Rac1. RESULTS:TIAM2 was highly expressed in NSCLC cells. Knockdown of TIAM2 inhibited the invasion and motility, and suppressed activation of Rac1. Further experiments demonstrated that knockdown of TIAM2 could up-regulate the expression of E-cadherin, and down- regulate the expression of MMP-3, Twist and Snail. CONCLUSIONS: Our data suggest that TIAM2 can promote invasion and motility of NSCLC cells. Activation of Rac1 and regulation of some EMT/invasion-related genes may be involved in the underlying processes.
Authors: Mariana Cooke; Gabriel Kreider-Letterman; Martin J Baker; Suli Zhang; Neil T Sullivan; Evgeniy Eruslanov; Martin C Abba; Silvia M Goicoechea; Rafael García-Mata; Marcelo G Kazanietz Journal: Cell Rep Date: 2021-11-02 Impact factor: 9.995
Authors: Anna Woroniuk; Andrew Porter; Gavin White; Daniel T Newman; Zoi Diamantopoulou; Thomas Waring; Claire Rooney; Douglas Strathdee; Daniel J Marston; Klaus M Hahn; Owen J Sansom; Tobias Zech; Angeliki Malliri Journal: Nat Commun Date: 2018-05-29 Impact factor: 14.919
Authors: J Amado-Azevedo; N R Reinhard; J van Bezu; R X de Menezes; V W van Beusechem; G P van Nieuw Amerongen; V W M van Hinsbergh; P L Hordijk Journal: Sci Rep Date: 2017-08-31 Impact factor: 4.379