Bo Tang1, Yi-Kuan Chen1, Wen-Jun Luo1, Jian Fu1, Jian-Ming Sun2. 1. Department of Vascular Surgery, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, PR China. 2. Department of Vascular Surgery, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, PR China. Electronic address: jianmingsun@live.cn.
Abstract
BACKGROUND: Deep venous thrombosis (DVT) and inflammation are two closely related entities. The objective of this study was to evaluate a possible association between interleukin-10 (IL-10) -1082A/G, -819C/T and -592C/A polymorphisms with DVT. METHODS: A case-control study was carried out in 660 patients with DVT and 660 age- and gender-matched healthy controls. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay was applied to identify the polymorphisms mentioned. RESULTS: Patients with DVT had a significantly lower frequency of IL-10 -1082GG genotype [odds ratio (OR)=0.59, 95% confidence interval (CI)=0.39, 0.89; P=0.01] than healthy controls. When stratifying by family history of DVT, it was found that patients with positive family history of DVT had a significantly lower frequency of IL-10 -1082GG genotype (OR=0.13, 95% CI=0.02, 0.95; P=0.04). When stratifying by smoking status, presence of varicose veins, type 2 diabetes mellitus and any hormone administration before, no significant differences were found in any groups. CONCLUSIONS: This study provides evidence that IL-10 -1082A/G polymorphism associated with risk of DVT. However, no association of the IL-10 -592C/A or -819C/T polymorphism with DVT risk was found. Additional well-designed large studies were required for the validation of our results.
BACKGROUND:Deep venous thrombosis (DVT) and inflammation are two closely related entities. The objective of this study was to evaluate a possible association between interleukin-10 (IL-10) -1082A/G, -819C/T and -592C/A polymorphisms with DVT. METHODS: A case-control study was carried out in 660 patients with DVT and 660 age- and gender-matched healthy controls. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay was applied to identify the polymorphisms mentioned. RESULTS:Patients with DVT had a significantly lower frequency of IL-10 -1082GG genotype [odds ratio (OR)=0.59, 95% confidence interval (CI)=0.39, 0.89; P=0.01] than healthy controls. When stratifying by family history of DVT, it was found that patients with positive family history of DVT had a significantly lower frequency of IL-10 -1082GG genotype (OR=0.13, 95% CI=0.02, 0.95; P=0.04). When stratifying by smoking status, presence of varicose veins, type 2 diabetes mellitus and any hormone administration before, no significant differences were found in any groups. CONCLUSIONS: This study provides evidence that IL-10-1082A/G polymorphism associated with risk of DVT. However, no association of the IL-10-592C/A or -819C/T polymorphism with DVT risk was found. Additional well-designed large studies were required for the validation of our results.
Authors: Knut A Mosevoll; Silje Johansen; Øystein Wendelbo; Ina Nepstad; Øystein Bruserud; Håkon Reikvam Journal: Front Med (Lausanne) Date: 2018-05-22