Lihua Liu1, Qin Dai, Zhigang Min, Ming Zhang. 1. Department of Neurology, First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710061, China. E-mail: chinaylh2003@163.com.
Abstract
OBJECTIVE: To investigate the effect of transforming growth factor β1 (TGF-β1) on the invasiveness of human glioma SF767 cell line in vitro and explore the possible molecular mechanism. METHODS: Human glioma SF767 cell line treated with TGF-β1 for 12, 24, or 48 h were examined for the expressions of epithelial-mesenchymal transition- and ERK/MAPK pathway-associated proteins using Western blotting. MTT assay and Transwell assay were employed to assess the changes in the cell proliferation and invasiveness after TGF-β1 treatment, respectively. RESULTS: Western blotting showed that TGF-β1 treatment up-regulated the expressions of vimentin, MMP-2, P-ERK, and Zeb-1 and down-regulated E-cadherin expression in SF767 cells. TGF-β1 treatment of the cells resulted in significantly shortened doubling time of cells and obviously increased cell invasiveness. CONCLUSION: TGF-β1 induces epithelial-mesenchymal transition of SF767 cell line to increase its invasiveness possibly via ERK/MAPK pathway.
OBJECTIVE: To investigate the effect of transforming growth factor β1 (TGF-β1) on the invasiveness of humanglioma SF767 cell line in vitro and explore the possible molecular mechanism. METHODS:Humanglioma SF767 cell line treated with TGF-β1 for 12, 24, or 48 h were examined for the expressions of epithelial-mesenchymal transition- and ERK/MAPK pathway-associated proteins using Western blotting. MTT assay and Transwell assay were employed to assess the changes in the cell proliferation and invasiveness after TGF-β1 treatment, respectively. RESULTS: Western blotting showed that TGF-β1 treatment up-regulated the expressions of vimentin, MMP-2, P-ERK, and Zeb-1 and down-regulated E-cadherin expression in SF767 cells. TGF-β1 treatment of the cells resulted in significantly shortened doubling time of cells and obviously increased cell invasiveness. CONCLUSION: TGF-β1 induces epithelial-mesenchymal transition of SF767 cell line to increase its invasiveness possibly via ERK/MAPK pathway.