BACKGROUND: The use of metal oxide nanoparticles (titanium dioxide) in consumer and industrial products improves their quality but also underscores the possible adverse effects to human and environmental health. MATERIALS & METHODS: Mice were exposed orally for 14 consecutive days and analyzed for alteration in different hepatic enzymes, histopathological changes, oxidative stress, DNA damage, tumor suppressor and proapoptotic protein expression in liver cells. RESULTS: We observed a significant alteration in the level of hepatic enzymes and liver histopathology at a dose of 100 mg/kg body weight. Significant oxidative DNA damage was observed in liver cells, which could be attributed to oxidative stress. In addition, the increased expression of p53, BAX, caspase-3 and -9 proteins and decreased expression of antiapoptotic protein Bcl-2, suggest activation of the intrinsic pathway of apoptosis. CONCLUSION: High accumulation of titanium dioxide nanoparticles in the liver tissue would cause DNA damage and apoptosis through the intrinsic pathway.
BACKGROUND: The use of metal oxide nanoparticles (titanium dioxide) in consumer and industrial products improves their quality but also underscores the possible adverse effects to human and environmental health. MATERIALS & METHODS:Mice were exposed orally for 14 consecutive days and analyzed for alteration in different hepatic enzymes, histopathological changes, oxidative stress, DNA damage, tumor suppressor and proapoptotic protein expression in liver cells. RESULTS: We observed a significant alteration in the level of hepatic enzymes and liver histopathology at a dose of 100 mg/kg body weight. Significant oxidative DNA damage was observed in liver cells, which could be attributed to oxidative stress. In addition, the increased expression of p53, BAX, caspase-3 and -9 proteins and decreased expression of antiapoptotic protein Bcl-2, suggest activation of the intrinsic pathway of apoptosis. CONCLUSION: High accumulation of titanium dioxide nanoparticles in the liver tissue would cause DNA damage and apoptosis through the intrinsic pathway.
Authors: Maged Younes; Gabriele Aquilina; Laurence Castle; Karl-Heinz Engel; Paul Fowler; Maria Jose Frutos Fernandez; Peter Fürst; Ursula Gundert-Remy; Rainer Gürtler; Trine Husøy; Melania Manco; Wim Mennes; Peter Moldeus; Sabina Passamonti; Romina Shah; Ine Waalkens-Berendsen; Detlef Wölfle; Emanuela Corsini; Francesco Cubadda; Didima De Groot; Rex FitzGerald; Sara Gunnare; Arno Christian Gutleb; Jan Mast; Alicja Mortensen; Agnes Oomen; Aldert Piersma; Veronika Plichta; Beate Ulbrich; Henk Van Loveren; Diane Benford; Margherita Bignami; Claudia Bolognesi; Riccardo Crebelli; Maria Dusinska; Francesca Marcon; Elsa Nielsen; Josef Schlatter; Christiane Vleminckx; Stefania Barmaz; Maria Carfí; Consuelo Civitella; Alessandra Giarola; Ana Maria Rincon; Rositsa Serafimova; Camilla Smeraldi; Jose Tarazona; Alexandra Tard; Matthew Wright Journal: EFSA J Date: 2021-05-06
Authors: Adriana Vieira; Ana Gramacho; Dora Rolo; Nádia Vital; Maria João Silva; Henriqueta Louro Journal: Adv Exp Med Biol Date: 2022 Impact factor: 2.622