E M Van Allen1, A Foye2, N Wagle1, W Kim2, S L Carter3, A McKenna4, J P Simko5, L A Garraway1, P G Febbo6. 1. 1] Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA [2] Cancer Program, Broad Institute of MIT and Harvard, Cambridge, MA, USA. 2. Department of Medicine, UCSF Helen Diller Family Comprehensive Cancer Center, San Francisco, CA, USA. 3. Cancer Program, Broad Institute of MIT and Harvard, Cambridge, MA, USA. 4. 1] Cancer Program, Broad Institute of MIT and Harvard, Cambridge, MA, USA [2] Department of Genome Sciences, University of Washington, Seattle, WA, USA. 5. 1] Department of Urology, UCSF Helen Diller Family Comprehensive Cancer Center, San Francisco, CA, USA [2] Department of Pathology, UCSF Helen Diller Family Comprehensive Cancer Center, San Francisco, CA, USA [3] Department of Radiation Oncology, UCSF Helen Diller Family Comprehensive Cancer Center, San Francisco, CA, USA. 6. 1] Department of Medicine, UCSF Helen Diller Family Comprehensive Cancer Center, San Francisco, CA, USA [2] Department of Urology, UCSF Helen Diller Family Comprehensive Cancer Center, San Francisco, CA, USA.
Abstract
BACKGROUND: Comprehensive molecular characterization of cancer that has metastasized to bone has proved challenging, which may limit the diagnostic and potential therapeutic opportunities for patients with bone-only metastatic disease. METHODS: We describe successful tissue acquisition, DNA extraction, and whole-exome sequencing from a bone metastasis of a patient with metastatic, castration-resistant prostate cancer (PCa). RESULTS: The resulting high-quality tumor sequencing identified plausibly actionable somatic genomic alterations that dysregulate the phosphoinostide 3-kinase pathway, as well as a theoretically actionable germline variant in the BRCA2 gene. CONCLUSIONS: We demonstrate the feasibility of diagnostic bone metastases profiling and analysis that will be required for the widespread application of prospective 'precision medicine' to men with advanced PCa.
BACKGROUND: Comprehensive molecular characterization of cancer that has metastasized to bone has proved challenging, which may limit the diagnostic and potential therapeutic opportunities for patients with bone-only metastatic disease. METHODS: We describe successful tissue acquisition, DNA extraction, and whole-exome sequencing from a bone metastasis of a patient with metastatic, castration-resistant prostate cancer (PCa). RESULTS: The resulting high-quality tumor sequencing identified plausibly actionable somatic genomic alterations that dysregulate the phosphoinostide 3-kinase pathway, as well as a theoretically actionable germline variant in the BRCA2 gene. CONCLUSIONS: We demonstrate the feasibility of diagnostic bone metastases profiling and analysis that will be required for the widespread application of prospective 'precision medicine' to men with advanced PCa.
Authors: Charles E Spritzer; P Diana Afonso; Emily N Vinson; James D Turnbull; Karla K Morris; Adam Foye; John F Madden; Kingshuk Roy Choudhury; Phillip G Febbo; Daniel J George Journal: Radiology Date: 2013-10-28 Impact factor: 11.105
Authors: Kristian Cibulskis; Aaron McKenna; Tim Fennell; Eric Banks; Mark DePristo; Gad Getz Journal: Bioinformatics Date: 2011-07-29 Impact factor: 6.937
Authors: J Chad Brenner; Bushra Ateeq; Yong Li; Anastasia K Yocum; Qi Cao; Irfan A Asangani; Sonam Patel; Xiaoju Wang; Hallie Liang; Jindan Yu; Nallasivam Palanisamy; Javed Siddiqui; Wei Yan; Xuhong Cao; Rohit Mehra; Aaron Sabolch; Venkatesha Basrur; Robert J Lonigro; Jun Yang; Scott A Tomlins; Christopher A Maher; Kojo S J Elenitoba-Johnson; Maha Hussain; Nora M Navone; Kenneth J Pienta; Sooryanarayana Varambally; Felix Y Feng; Arul M Chinnaiyan Journal: Cancer Cell Date: 2011-05-17 Impact factor: 31.743
Authors: Johann S de Bono; Christopher J Logothetis; Arturo Molina; Karim Fizazi; Scott North; Luis Chu; Kim N Chi; Robert J Jones; Oscar B Goodman; Fred Saad; John N Staffurth; Paul Mainwaring; Stephen Harland; Thomas W Flaig; Thomas E Hutson; Tina Cheng; Helen Patterson; John D Hainsworth; Charles J Ryan; Cora N Sternberg; Susan L Ellard; Aude Fléchon; Mansoor Saleh; Mark Scholz; Eleni Efstathiou; Andrea Zivi; Diletta Bianchini; Yohann Loriot; Nicole Chieffo; Thian Kheoh; Christopher M Haqq; Howard I Scher Journal: N Engl J Med Date: 2011-05-26 Impact factor: 91.245
Authors: Rohit Mehra; Chandan Kumar-Sinha; Sunita Shankar; Robert J Lonigro; Xiaojun Jing; Neena E Philips; Javed Siddiqui; Bo Han; Xuhong Cao; David C Smith; Rajal B Shah; Arul M Chinnaiyan; Kenneth J Pienta Journal: Clin Cancer Res Date: 2011-05-09 Impact factor: 12.531
Authors: Sameek Roychowdhury; Matthew K Iyer; Dan R Robinson; Robert J Lonigro; Yi-Mi Wu; Xuhong Cao; Shanker Kalyana-Sundaram; Lee Sam; O Alejandro Balbin; Michael J Quist; Terrence Barrette; Jessica Everett; Javed Siddiqui; Lakshmi P Kunju; Nora Navone; John C Araujo; Patricia Troncoso; Christopher J Logothetis; Jeffrey W Innis; David C Smith; Christopher D Lao; Scott Y Kim; J Scott Roberts; Stephen B Gruber; Kenneth J Pienta; Moshe Talpaz; Arul M Chinnaiyan Journal: Sci Transl Med Date: 2011-11-30 Impact factor: 17.956
Authors: Sheila Fisher; Andrew Barry; Justin Abreu; Brian Minie; Jillian Nolan; Toni M Delorey; Geneva Young; Timothy J Fennell; Alexander Allen; Lauren Ambrogio; Aaron M Berlin; Brendan Blumenstiel; Kristian Cibulskis; Dennis Friedrich; Ryan Johnson; Frank Juhn; Brian Reilly; Ramy Shammas; John Stalker; Sean M Sykes; Jon Thompson; John Walsh; Andrew Zimmer; Zac Zwirko; Stacey Gabriel; Robert Nicol; Chad Nusbaum Journal: Genome Biol Date: 2011-01-04 Impact factor: 13.583
Authors: Brett S Carver; Caren Chapinski; John Wongvipat; Haley Hieronymus; Yu Chen; Sarat Chandarlapaty; Vivek K Arora; Carl Le; Jason Koutcher; Howard Scher; Peter T Scardino; Neal Rosen; Charles L Sawyers Journal: Cancer Cell Date: 2011-05-17 Impact factor: 31.743
Authors: Andrea K Miyahira; Adam Sharp; Leigh Ellis; Jennifer Jones; Salma Kaochar; H Benjamin Larman; David A Quigley; Huihui Ye; Jonathan W Simons; Kenneth J Pienta; Howard R Soule Journal: Prostate Date: 2019-12-11 Impact factor: 4.104
Authors: Verena Sailer; Marc H Schiffman; Myriam Kossai; Joanna Cyrta; Shaham Beg; Brian Sullivan; Bradley B Pua; Kyungmouk Steve Lee; Adam D Talenfeld; David M Nanus; Scott T Tagawa; Brian D Robinson; Rema A Rao; Chantal Pauli; Rohan Bareja; Luis S Beltran; Alexandros Sigaras; Kenneth Wa Eng; Olivier Elemento; Andrea Sboner; Mark A Rubin; Himisha Beltran; Juan Miguel Mosquera Journal: Cancer Date: 2017-12-19 Impact factor: 6.860
Authors: Dan Robinson; Eliezer M Van Allen; Yi-Mi Wu; Nikolaus Schultz; Robert J Lonigro; Juan-Miguel Mosquera; Bruce Montgomery; Mary-Ellen Taplin; Colin C Pritchard; Gerhardt Attard; Himisha Beltran; Wassim Abida; Robert K Bradley; Jake Vinson; Xuhong Cao; Pankaj Vats; Lakshmi P Kunju; Maha Hussain; Felix Y Feng; Scott A Tomlins; Kathleen A Cooney; David C Smith; Christine Brennan; Javed Siddiqui; Rohit Mehra; Yu Chen; Dana E Rathkopf; Michael J Morris; Stephen B Solomon; Jeremy C Durack; Victor E Reuter; Anuradha Gopalan; Jianjiong Gao; Massimo Loda; Rosina T Lis; Michaela Bowden; Stephen P Balk; Glenn Gaviola; Carrie Sougnez; Manaswi Gupta; Evan Y Yu; Elahe A Mostaghel; Heather H Cheng; Hyojeong Mulcahy; Lawrence D True; Stephen R Plymate; Heidi Dvinge; Roberta Ferraldeschi; Penny Flohr; Susana Miranda; Zafeiris Zafeiriou; Nina Tunariu; Joaquin Mateo; Raquel Perez-Lopez; Francesca Demichelis; Brian D Robinson; Marc Schiffman; David M Nanus; Scott T Tagawa; Alexandros Sigaras; Kenneth W Eng; Olivier Elemento; Andrea Sboner; Elisabeth I Heath; Howard I Scher; Kenneth J Pienta; Philip Kantoff; Johann S de Bono; Mark A Rubin; Peter S Nelson; Levi A Garraway; Charles L Sawyers; Arul M Chinnaiyan Journal: Cell Date: 2015-05-21 Impact factor: 41.582
Authors: S Gillessen; A Omlin; G Attard; J S de Bono; E Efstathiou; K Fizazi; S Halabi; P S Nelson; O Sartor; M R Smith; H R Soule; H Akaza; T M Beer; H Beltran; A M Chinnaiyan; G Daugaard; I D Davis; M De Santis; C G Drake; R A Eeles; S Fanti; M E Gleave; A Heidenreich; M Hussain; N D James; F E Lecouvet; C J Logothetis; K Mastris; S Nilsson; W K Oh; D Olmos; A R Padhani; C Parker; M A Rubin; J A Schalken; H I Scher; A Sella; N D Shore; E J Small; C N Sternberg; H Suzuki; C J Sweeney; I F Tannock; B Tombal Journal: Ann Oncol Date: 2015-06-03 Impact factor: 32.976
Authors: Danielle Mercatante Carrick; Michele G Mehaffey; Michael C Sachs; Sean Altekruse; Corinne Camalier; Rodrigo Chuaqui; Wendy Cozen; Biswajit Das; Brenda Y Hernandez; Chih-Jian Lih; Charles F Lynch; Hala Makhlouf; Paul McGregor; Lisa M McShane; JoyAnn Phillips Rohan; William D Walsh; Paul M Williams; Elizabeth M Gillanders; Leah E Mechanic; Sheri D Schully Journal: PLoS One Date: 2015-07-29 Impact factor: 3.240
Authors: Alan F Scott; David W Mohr; Hua Ling; Robert B Scharpf; Peng Zhang; Gregory S Liptak Journal: Genes (Basel) Date: 2014-05-12 Impact factor: 4.096