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Literature DB >> 2436507

Rapid purification of DNA from agarose gels by centrifugation through a disposable plastic column.

Abstract

A simple and rapid method for purifying DNA from agarose gels is described. Agarose slices containing DNA are placed in a disposable plastic column and the DNA is separated from the agarose by centrifugation in a microfuge. Recoveries averaging 25% are obtained for DNA of 14 kb or less. The recovered DNA can be labeled to high specific activity, cleaved with restriction endonucleases, and ligated efficiently using standard cloning vectors.

Entities: Chemical

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Year: 1987 PMID： 2436507 DOI： 10.1016/0003-2697(87)90620-8

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

8 in total

1. Karyoplasmic interaction selection strategy: a general strategy to detect protein-protein interactions in mammalian cells.

Authors: E R Fearon; T Finkel; M L Gillison; S P Kennedy; J F Casella; G F Tomaselli; J S Morrow; C Van Dang
Journal: Proc Natl Acad Sci U S A Date: 1992-09-01 Impact factor: 11.205

2. GLI3 encodes a 190-kilodalton protein with multiple regions of GLI similarity.

Authors: J M Ruppert; B Vogelstein; K Arheden; K W Kinzler
Journal: Mol Cell Biol Date: 1990-10 Impact factor: 4.272

3. The GLI-Kruppel family of human genes.

Authors: J M Ruppert; K W Kinzler; A J Wong; S H Bigner; F T Kao; M L Law; H N Seuanez; S J O'Brien; B Vogelstein
Journal: Mol Cell Biol Date: 1988-08 Impact factor: 4.272

4. Sequence analysis and characterization of pOM1, a small cryptic plasmid from Butyrivibrio fibrisolvens, and its use in construction of a new family of cloning vectors for Butyrivibrios.

Authors: M A Hefford; Y Kobayashi; S E Allard; R J Forster; R M Teather
Journal: Appl Environ Microbiol Date: 1997-05 Impact factor: 4.792

5. Hair-specific expression of chloramphenicol acetyltransferase in transgenic mice under the control of an ultra-high-sulfur keratin promoter.

Authors: A R McNab; P Andrus; T E Wagner; A E Buhl; D J Waldon; T T Kawabe; T J Rea; V Groppi; G Vogeli
Journal: Proc Natl Acad Sci U S A Date: 1990-09 Impact factor: 11.205

Rapid purification of DNA from agarose gels by centrifugation through a disposable plastic column.

1. Karyoplasmic interaction selection strategy: a general strategy to detect protein-protein interactions in mammalian cells.

2. GLI3 encodes a 190-kilodalton protein with multiple regions of GLI similarity.

3. The GLI-Kruppel family of human genes.

4. Sequence analysis and characterization of pOM1, a small cryptic plasmid from Butyrivibrio fibrisolvens, and its use in construction of a new family of cloning vectors for Butyrivibrios.

5. Hair-specific expression of chloramphenicol acetyltransferase in transgenic mice under the control of an ultra-high-sulfur keratin promoter.

6. In vivo cloning of PCR products in E. coli.

7. Characterization of N-myc amplification units in human neuroblastoma cells.

8. ACT3: a putative centractin homologue in S. cerevisiae is required for proper orientation of the mitotic spindle.