| Literature DB >> 24365042 |
Zrinka Stritof Majetic1, Renee Galloway2, Eva Ruzic Sabljic3, Zoran Milas4, Vesna Mojcec Perko4, Josipa Habus4, Josip Margaletic5, Renata Pernar5, Nenad Turk4.
Abstract
In this survey we investigated a population of small mammals in Eastern Croatia in order to determine Leptospira carriage rates and identify circulating serovars. Out of 67 trapped animals, 20 (29.9%) isolates were obtained. Identification of isolates using microscopic agglutination test, pulsed field gel electrophoresis and multi locus sequence typing revealed that 10 (50.0%) isolates belong to serogroup Pomona, serovar Mozdok, 6 (30.0%) isolates to serogroup Australis, serovar Jalna, 2 (10.0%) isolates to serogroup Sejroe, serovar Saxkoebing, and 1 (5.0%) isolate to serogroup Grippotyphosa, serovar Grippotyphosa. One isolate from serogroup Bataviae was unable to be identified to the serovar level. Amplification of a 331-bp region of the locus LA0322 using real-time polymerase chain reaction determined that 12 (60.0%) isolates belong to L. kirschneri, 6 (30.0%) isolates to L. interrogans, and 2 (10.0%) isolates to L. borgpetersenii. Leptospira carriage rate was high (29.9%), which corresponds to a high incidence of human and domestic animal leptospirosis in Eastern Croatia. Furthermore, 90.0% of the isolates belong to serogroups Pomona, Australis and Sejroe which are also the most prevalent serogroups in humans in this area. These findings suggest that small mammals might be an important source of Leptospira spp. infection in Eastern Croatia.Entities:
Keywords: Leptospira; Leptospirosis; Molecular; Reservoirs; Small mammals; Zoonosis
Mesh：
Year: 2013 PMID： 24365042 PMCID： PMC6374769 DOI： 10.1016/j.actatropica.2013.12.009
Source DB: PubMed Journal: Acta Trop ISSN： 0001-706X Impact factor: 3.112